Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency

BACKGROUND: Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or modify target DNA cassettes. One important metabolic engineering strategy is to l...

Descripción completa

Detalles Bibliográficos
Autores principales: Xie, Qianwen, Li, Siwei, Zhao, Dongdong, Ye, Lijun, Li, Qingyan, Zhang, Xueli, Zhu, Li, Bi, Changhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737257/
https://www.ncbi.nlm.nih.gov/pubmed/33317552
http://dx.doi.org/10.1186/s12934-020-01496-w

Ejemplares similares

Ejemplares similares