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β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study

Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with  intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first...

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Autores principales: Abdelrahman, Dina N., Taha, Aya A., Dafaallah, Mazar M., Mohammed, Alaa Abdelgafoor, El Hussein, Abdel Rahim M., Hashim, Ahmed I., Hamedelnil, Yousif F., Altayb, Hisham N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737708/
https://www.ncbi.nlm.nih.gov/pubmed/33363717
http://dx.doi.org/10.12688/f1000research.24818.3
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author Abdelrahman, Dina N.
Taha, Aya A.
Dafaallah, Mazar M.
Mohammed, Alaa Abdelgafoor
El Hussein, Abdel Rahim M.
Hashim, Ahmed I.
Hamedelnil, Yousif F.
Altayb, Hisham N.
author_facet Abdelrahman, Dina N.
Taha, Aya A.
Dafaallah, Mazar M.
Mohammed, Alaa Abdelgafoor
El Hussein, Abdel Rahim M.
Hashim, Ahmed I.
Hamedelnil, Yousif F.
Altayb, Hisham N.
author_sort Abdelrahman, Dina N.
collection PubMed
description Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with  intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes ( blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant to Imipenem while all isolates were resistant to Amoxicillin and Amoxyclav (100%). A total of 43 (54%) Ps. aeruginosa isolates were positive for blaTEM, blaSHV, blaCTXM-1, blaVEB and blaOXA-1 genes, while 27 (34%) were positive for class C β- Lactamases, and 20 (25%) were positive for both classes. Frequency of beta-lactamases genes was as follows: blaTEM, 19 (44.2%); blaSHV, 16 (37.2%); bla CTX-M1, 10 (23.3%); blaVEB, 14 (32.6%); blaOXA-1, 7 (16.3%). blaAmpC 22 (81.5%) and bla DHA 8 (29.6%).  In total, 3 (11.1%) isolates were positive for both bla AmpC and blaDHA genes. Conclusion: Ps. aeruginosa isolates showed a high rate of β- lactamases production, with co-resistance to other antibiotic classes. The lowest resistance rate of Ps. aeruginosa was to Imipenem followed by Gentamicin and Ciprofloxacin. No statistically significant relationship between production of β-lactamases in Ps. aeruginosa and resistance to third generation cephalosporins was found.
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spelling pubmed-77377082020-12-23 β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study Abdelrahman, Dina N. Taha, Aya A. Dafaallah, Mazar M. Mohammed, Alaa Abdelgafoor El Hussein, Abdel Rahim M. Hashim, Ahmed I. Hamedelnil, Yousif F. Altayb, Hisham N. F1000Res Research Article Background: Pseudomonas aeruginosa is a pathogenic bacterium, causing nosocomial infections with  intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases genes frequency in Ps. aeruginosa in Khartoum State, Sudan. Methods: 121 Ps. aeruginosa clinical isolates from various clinical specimens were used in this cross sectional study conducted in Khartoum State. Eighty isolates were confirmed as Ps. aeruginosa through conventional identification methods and species specific primers. The susceptibility pattern of the confirmed isolates to selected antibiotics was done following the Kirby Bauer disk diffusion method. Multiplex PCR was used for detection of seven β-lactamase genes ( blaTEM, blaSHV, blaCTXM-1, blaVEB, blaOXA-1, blaAmpC and blaDHA). Results: Of the 80 confirmed Ps. aeruginosa isolates, 8 (10%) were resistant to Imipenem while all isolates were resistant to Amoxicillin and Amoxyclav (100%). A total of 43 (54%) Ps. aeruginosa isolates were positive for blaTEM, blaSHV, blaCTXM-1, blaVEB and blaOXA-1 genes, while 27 (34%) were positive for class C β- Lactamases, and 20 (25%) were positive for both classes. Frequency of beta-lactamases genes was as follows: blaTEM, 19 (44.2%); blaSHV, 16 (37.2%); bla CTX-M1, 10 (23.3%); blaVEB, 14 (32.6%); blaOXA-1, 7 (16.3%). blaAmpC 22 (81.5%) and bla DHA 8 (29.6%).  In total, 3 (11.1%) isolates were positive for both bla AmpC and blaDHA genes. Conclusion: Ps. aeruginosa isolates showed a high rate of β- lactamases production, with co-resistance to other antibiotic classes. The lowest resistance rate of Ps. aeruginosa was to Imipenem followed by Gentamicin and Ciprofloxacin. No statistically significant relationship between production of β-lactamases in Ps. aeruginosa and resistance to third generation cephalosporins was found. F1000 Research Limited 2021-04-01 /pmc/articles/PMC7737708/ /pubmed/33363717 http://dx.doi.org/10.12688/f1000research.24818.3 Text en Copyright: © 2021 Abdelrahman DN et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abdelrahman, Dina N.
Taha, Aya A.
Dafaallah, Mazar M.
Mohammed, Alaa Abdelgafoor
El Hussein, Abdel Rahim M.
Hashim, Ahmed I.
Hamedelnil, Yousif F.
Altayb, Hisham N.
β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title_full β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title_fullStr β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title_full_unstemmed β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title_short β-lactamases (bla (TEM), bla (SHV), bla (CTXM-1), bla (VEB), bla (OXA-1)) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study
title_sort β-lactamases (bla (tem), bla (shv), bla (ctxm-1), bla (veb), bla (oxa-1)) and class c β-lactamases gene frequency in pseudomonas aeruginosa isolated from various clinical specimens in khartoum state, sudan: a cross sectional study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737708/
https://www.ncbi.nlm.nih.gov/pubmed/33363717
http://dx.doi.org/10.12688/f1000research.24818.3
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