Toward a better definition of hematopoietic progenitors suitable for B cell differentiation

The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B cells exert roles as effector and as regulator of immune responses in different clinical settings, we were interested in generating B cells from hIPSC. We differentiated human embryo...

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Detalles Bibliográficos
Autores principales: Dubois, Florian, Gaignerie, Anne, Flippe, Léa, Heslan, Jean-Marie, Tesson, Laurent, Chesneau, Mélanie, Haspot, Fabienne, Conchon, Sophie, David, Laurent, Brouard, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7737978/
https://www.ncbi.nlm.nih.gov/pubmed/33320872
http://dx.doi.org/10.1371/journal.pone.0243769
Descripción
Sumario:The success of inducing human pluripotent stem cells (hIPSC) offers new opportunities for cell-based therapy. Since B cells exert roles as effector and as regulator of immune responses in different clinical settings, we were interested in generating B cells from hIPSC. We differentiated human embryonic stem cells (hESC) and hIPSC into B cells onto OP9 and MS-5 stromal cells successively. We overcame issues in generating CD34(+)CD43(+) hematopoietic progenitors with appropriate cytokine conditions and emphasized the difficulties to generate proper hematopoietic progenitors. We highlight CD31(int)CD45(int) phenotype as a possible marker of hematopoietic progenitors suitable for B cell differentiation. Defining precisely proper lymphoid progenitors will improve the study of their lineage commitment and the signals needed during the in vitro process.

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