Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues

Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence the evolutionary trajectory, an aspect that is often overlooked in laboratory directed evolution studies. We selected the Escherichia coli nit...

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Detalles Bibliográficos
Autores principales: Hall, Kelsi R, Robins, Katherine J, Williams, Elsie M, Rich, Michelle H, Calcott, Mark J, Copp, Janine N, Little, Rory F, Schwörer, Ralf, Evans, Gary B, Patrick, Wayne M, Ackerley, David F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738182/
https://www.ncbi.nlm.nih.gov/pubmed/33185191
http://dx.doi.org/10.7554/eLife.59081

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738182/
https://www.ncbi.nlm.nih.gov/pubmed/33185191
http://dx.doi.org/10.7554/eLife.59081

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