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Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues
Selection for a promiscuous enzyme activity provides substantial opportunity for competition between endogenous and newly-encountered substrates to influence the evolutionary trajectory, an aspect that is often overlooked in laboratory directed evolution studies. We selected the Escherichia coli nit...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
eLife Sciences Publications, Ltd
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738182/ https://www.ncbi.nlm.nih.gov/pubmed/33185191 http://dx.doi.org/10.7554/eLife.59081 |