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“Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”

In vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (...

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Autores principales: Saliba, Wilson Pardini, Gimenes, Lindsay Unno, Drumond, Roberti Martins, Bayão, Henrique Xavier Salgado, Di Palo, Rossella, Gasparrini, Bianca, Rubessa, Marcello, Baruselli, Pietro Sampaio, Sales, José Nélio Souza, Bastianetto, Eduardo, Leite, Rômulo Cerqueira, Alvim, Mucio Túlio Teixeira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738327/
https://www.ncbi.nlm.nih.gov/pubmed/33344525
http://dx.doi.org/10.3389/fvets.2020.577775
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author Saliba, Wilson Pardini
Gimenes, Lindsay Unno
Drumond, Roberti Martins
Bayão, Henrique Xavier Salgado
Di Palo, Rossella
Gasparrini, Bianca
Rubessa, Marcello
Baruselli, Pietro Sampaio
Sales, José Nélio Souza
Bastianetto, Eduardo
Leite, Rômulo Cerqueira
Alvim, Mucio Túlio Teixeira
author_facet Saliba, Wilson Pardini
Gimenes, Lindsay Unno
Drumond, Roberti Martins
Bayão, Henrique Xavier Salgado
Di Palo, Rossella
Gasparrini, Bianca
Rubessa, Marcello
Baruselli, Pietro Sampaio
Sales, José Nélio Souza
Bastianetto, Eduardo
Leite, Rômulo Cerqueira
Alvim, Mucio Túlio Teixeira
author_sort Saliba, Wilson Pardini
collection PubMed
description In vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (P/ET), pregnancy loss (PL), and calving rates in buffalo recipients. The studied factors were season, recipient parity, the synchronization protocol, the CL diameter, asynchrony between the embryo and the recipient, the day of the recipient estrous cycle, the embryo (fresh vs. vitrified), the day of embryo development, and the embryo stage. These retrospective data, from a program of in vitro produced embryos, were analyzed by logistic regression, and the odds ratio was also estimated. Two factors were related to P/ET and the calving rate: (1) progesterone associated to estradiol plus eCG protocol for fixed time ET tended to affect positively P/ET on day 30 (41.9 vs. 36.1%, respectively; P = 0.07; AOR = 1.28) and P/ET on day 60 (37.8 vs. 36.1%, respectively; P = 0.09; AOR = 1.08) compared to the Ovsynch protocol; and (2) the CL diameter (≥14.5 mm) at transfer increased P/ET on day 30 (47.4 vs. 32.5%; P < 0.01; AOR = 1.87) and on day 60 (45.3 vs. 27.7%; P < 0.01; AOR = 2.16), and also the calving rate (37.9 vs. 21.7%; P < 0.01; AOR = 2.20). PL was greater when ET was done in the nonbreeding season compared to the breeding season (PL 30–60: 12.8 vs. 0.0%, P = 0.01; AOR > 999.99; PL 60-calving: 26.8 vs. 3.6%, P = 0.03; AOR = 9.90; and PL 30-calving: 36.2 vs. 3.6%, P = 0.01; AOR = 15.30). In conclusion, the data of our study indicated that the synchronization protocol, the CL diameter, and ET during the breeding season impacted the reproductive efficiency of buffalo recipients.
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spelling pubmed-77383272020-12-17 “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?” Saliba, Wilson Pardini Gimenes, Lindsay Unno Drumond, Roberti Martins Bayão, Henrique Xavier Salgado Di Palo, Rossella Gasparrini, Bianca Rubessa, Marcello Baruselli, Pietro Sampaio Sales, José Nélio Souza Bastianetto, Eduardo Leite, Rômulo Cerqueira Alvim, Mucio Túlio Teixeira Front Vet Sci Veterinary Science In vitro embryo production and embryo transfer (ET) in buffaloes has been developed for decades. However, most studies are focused on the donor or laboratory improvements, and there is a lack of reports regarding the recipients. Therefore, our aim was to investigate factors associated to pregnancy (P/ET), pregnancy loss (PL), and calving rates in buffalo recipients. The studied factors were season, recipient parity, the synchronization protocol, the CL diameter, asynchrony between the embryo and the recipient, the day of the recipient estrous cycle, the embryo (fresh vs. vitrified), the day of embryo development, and the embryo stage. These retrospective data, from a program of in vitro produced embryos, were analyzed by logistic regression, and the odds ratio was also estimated. Two factors were related to P/ET and the calving rate: (1) progesterone associated to estradiol plus eCG protocol for fixed time ET tended to affect positively P/ET on day 30 (41.9 vs. 36.1%, respectively; P = 0.07; AOR = 1.28) and P/ET on day 60 (37.8 vs. 36.1%, respectively; P = 0.09; AOR = 1.08) compared to the Ovsynch protocol; and (2) the CL diameter (≥14.5 mm) at transfer increased P/ET on day 30 (47.4 vs. 32.5%; P < 0.01; AOR = 1.87) and on day 60 (45.3 vs. 27.7%; P < 0.01; AOR = 2.16), and also the calving rate (37.9 vs. 21.7%; P < 0.01; AOR = 2.20). PL was greater when ET was done in the nonbreeding season compared to the breeding season (PL 30–60: 12.8 vs. 0.0%, P = 0.01; AOR > 999.99; PL 60-calving: 26.8 vs. 3.6%, P = 0.03; AOR = 9.90; and PL 30-calving: 36.2 vs. 3.6%, P = 0.01; AOR = 15.30). In conclusion, the data of our study indicated that the synchronization protocol, the CL diameter, and ET during the breeding season impacted the reproductive efficiency of buffalo recipients. Frontiers Media S.A. 2020-12-02 /pmc/articles/PMC7738327/ /pubmed/33344525 http://dx.doi.org/10.3389/fvets.2020.577775 Text en Copyright © 2020 Saliba, Gimenes, Drumond, Bayão, Di Palo, Gasparrini, Rubessa, Baruselli, Sales, Bastianetto, Leite and Alvim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Saliba, Wilson Pardini
Gimenes, Lindsay Unno
Drumond, Roberti Martins
Bayão, Henrique Xavier Salgado
Di Palo, Rossella
Gasparrini, Bianca
Rubessa, Marcello
Baruselli, Pietro Sampaio
Sales, José Nélio Souza
Bastianetto, Eduardo
Leite, Rômulo Cerqueira
Alvim, Mucio Túlio Teixeira
“Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title_full “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title_fullStr “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title_full_unstemmed “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title_short “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”
title_sort “which factors affect pregnancy until calving and pregnancy loss in buffalo recipients of in vitro produced embryos?”
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738327/
https://www.ncbi.nlm.nih.gov/pubmed/33344525
http://dx.doi.org/10.3389/fvets.2020.577775
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