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Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation

In flowering plants, sugars act as carbon sources providing energy for developing embryos and seeds. Although most studies focus on carbon metabolism in whole seeds, knowledge about how particular sugars contribute to the developmental transitions during embryogenesis is scarce. To develop a quantit...

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Autores principales: Moreno Curtidor, Catalina, Annunziata, Maria Grazia, Gupta, Saurabh, Apelt, Federico, Richard, Sarah Isabel, Kragler, Friedrich, Mueller-Roeber, Bernd, Olas, Justyna Jadwiga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738343/
https://www.ncbi.nlm.nih.gov/pubmed/33343596
http://dx.doi.org/10.3389/fpls.2020.588433
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author Moreno Curtidor, Catalina
Annunziata, Maria Grazia
Gupta, Saurabh
Apelt, Federico
Richard, Sarah Isabel
Kragler, Friedrich
Mueller-Roeber, Bernd
Olas, Justyna Jadwiga
author_facet Moreno Curtidor, Catalina
Annunziata, Maria Grazia
Gupta, Saurabh
Apelt, Federico
Richard, Sarah Isabel
Kragler, Friedrich
Mueller-Roeber, Bernd
Olas, Justyna Jadwiga
author_sort Moreno Curtidor, Catalina
collection PubMed
description In flowering plants, sugars act as carbon sources providing energy for developing embryos and seeds. Although most studies focus on carbon metabolism in whole seeds, knowledge about how particular sugars contribute to the developmental transitions during embryogenesis is scarce. To develop a quantitative understanding of how carbon composition changes during embryo development, and to determine how sugar status contributes to final seed or embryo size, we performed metabolic profiling of hand-dissected embryos at late torpedo and mature stages, and dormant seeds, in two Arabidopsis thaliana accessions with medium [Columbia-0 (Col-0)] and large [Burren-0 (Bur-0)] seed sizes, respectively. Our results show that, in both accessions, metabolite profiles of embryos largely differ from those of dormant seeds. We found that developmental transitions from torpedo to mature embryos, and further to dormant seeds, are associated with major metabolic switches in carbon reserve accumulation. While glucose, sucrose, and starch predominantly accumulated during seed dormancy, fructose levels were strongly elevated in mature embryos. Interestingly, Bur-0 seeds contain larger mature embryos than Col-0 seeds. Fructose and starch were accumulated to significantly higher levels in mature Bur-0 than Col-0 embryos, suggesting that they contribute to the enlarged mature Bur-0 embryos. Furthermore, we found that Bur-0 embryos accumulated a higher level of sucrose compared to hexose sugars and that changes in sucrose metabolism are mediated by sucrose synthase (SUS), with SUS genes acting non-redundantly, and in a tissue-specific manner to utilize sucrose during late embryogenesis.
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spelling pubmed-77383432020-12-17 Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation Moreno Curtidor, Catalina Annunziata, Maria Grazia Gupta, Saurabh Apelt, Federico Richard, Sarah Isabel Kragler, Friedrich Mueller-Roeber, Bernd Olas, Justyna Jadwiga Front Plant Sci Plant Science In flowering plants, sugars act as carbon sources providing energy for developing embryos and seeds. Although most studies focus on carbon metabolism in whole seeds, knowledge about how particular sugars contribute to the developmental transitions during embryogenesis is scarce. To develop a quantitative understanding of how carbon composition changes during embryo development, and to determine how sugar status contributes to final seed or embryo size, we performed metabolic profiling of hand-dissected embryos at late torpedo and mature stages, and dormant seeds, in two Arabidopsis thaliana accessions with medium [Columbia-0 (Col-0)] and large [Burren-0 (Bur-0)] seed sizes, respectively. Our results show that, in both accessions, metabolite profiles of embryos largely differ from those of dormant seeds. We found that developmental transitions from torpedo to mature embryos, and further to dormant seeds, are associated with major metabolic switches in carbon reserve accumulation. While glucose, sucrose, and starch predominantly accumulated during seed dormancy, fructose levels were strongly elevated in mature embryos. Interestingly, Bur-0 seeds contain larger mature embryos than Col-0 seeds. Fructose and starch were accumulated to significantly higher levels in mature Bur-0 than Col-0 embryos, suggesting that they contribute to the enlarged mature Bur-0 embryos. Furthermore, we found that Bur-0 embryos accumulated a higher level of sucrose compared to hexose sugars and that changes in sucrose metabolism are mediated by sucrose synthase (SUS), with SUS genes acting non-redundantly, and in a tissue-specific manner to utilize sucrose during late embryogenesis. Frontiers Media S.A. 2020-12-02 /pmc/articles/PMC7738343/ /pubmed/33343596 http://dx.doi.org/10.3389/fpls.2020.588433 Text en Copyright © 2020 Moreno Curtidor, Annunziata, Gupta, Apelt, Richard, Kragler, Mueller-Roeber and Olas. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Moreno Curtidor, Catalina
Annunziata, Maria Grazia
Gupta, Saurabh
Apelt, Federico
Richard, Sarah Isabel
Kragler, Friedrich
Mueller-Roeber, Bernd
Olas, Justyna Jadwiga
Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title_full Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title_fullStr Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title_full_unstemmed Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title_short Physiological Profiling of Embryos and Dormant Seeds in Two Arabidopsis Accessions Reveals a Metabolic Switch in Carbon Reserve Accumulation
title_sort physiological profiling of embryos and dormant seeds in two arabidopsis accessions reveals a metabolic switch in carbon reserve accumulation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738343/
https://www.ncbi.nlm.nih.gov/pubmed/33343596
http://dx.doi.org/10.3389/fpls.2020.588433
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