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Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids
The 80% mortality rate of pancreatic-cancer (PC) makes early diagnosis a challenge. Oral fluids (OF) may be considered the ultimate body fluid for non-invasive examinations. We have developed techniques to improve visualization of minor OF proteins thereby overcoming major barriers to using OF as a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738525/ https://www.ncbi.nlm.nih.gov/pubmed/33319845 http://dx.doi.org/10.1038/s41598-020-78922-x |
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author | Deutsch, O. Haviv, Y. Krief, G. Keshet, N. Westreich, R. Stemmer, S. M. Zaks, B. Navat, S. P. Yanko, R. Lahav, O. Aframian, D. J. Palmon, A. |
author_facet | Deutsch, O. Haviv, Y. Krief, G. Keshet, N. Westreich, R. Stemmer, S. M. Zaks, B. Navat, S. P. Yanko, R. Lahav, O. Aframian, D. J. Palmon, A. |
author_sort | Deutsch, O. |
collection | PubMed |
description | The 80% mortality rate of pancreatic-cancer (PC) makes early diagnosis a challenge. Oral fluids (OF) may be considered the ultimate body fluid for non-invasive examinations. We have developed techniques to improve visualization of minor OF proteins thereby overcoming major barriers to using OF as a diagnostic fluid. The aim of this study was to establish a short discriminative panel of OF biomarkers for the detection of PC. Unstimulated OF were collected from PC patients and controls (n = 30). High-abundance-proteins were depleted and the remaining proteins were analyzed by two-dimensional-gel-electrophoresis and quantitative dimethylation-liquid-chromatography-tandem mass-spectrometry. Label-free quantitative-mass-spectrometry analysis (qMS) was performed on 20 individual samples (n = 20). More than 100 biomarker candidates were identified in OF samples, and 21 had a highly differential expression profile. qMS analysis yielded a ROC-plot AUC value of 0.91 with 90.0% sensitivity and specificity for a combination of five biomarker candidates. We found a combination of five biomarkers for PC. Most of these proteins are known to be related to PC or other gastric cancers, but have never been detected in OF. This study demonstrates the importance of novel OF depletion methodologies for increased protein visibility and highlights the clinical applicability of OF as a diagnostic fluid. |
format | Online Article Text |
id | pubmed-7738525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77385252020-12-17 Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids Deutsch, O. Haviv, Y. Krief, G. Keshet, N. Westreich, R. Stemmer, S. M. Zaks, B. Navat, S. P. Yanko, R. Lahav, O. Aframian, D. J. Palmon, A. Sci Rep Article The 80% mortality rate of pancreatic-cancer (PC) makes early diagnosis a challenge. Oral fluids (OF) may be considered the ultimate body fluid for non-invasive examinations. We have developed techniques to improve visualization of minor OF proteins thereby overcoming major barriers to using OF as a diagnostic fluid. The aim of this study was to establish a short discriminative panel of OF biomarkers for the detection of PC. Unstimulated OF were collected from PC patients and controls (n = 30). High-abundance-proteins were depleted and the remaining proteins were analyzed by two-dimensional-gel-electrophoresis and quantitative dimethylation-liquid-chromatography-tandem mass-spectrometry. Label-free quantitative-mass-spectrometry analysis (qMS) was performed on 20 individual samples (n = 20). More than 100 biomarker candidates were identified in OF samples, and 21 had a highly differential expression profile. qMS analysis yielded a ROC-plot AUC value of 0.91 with 90.0% sensitivity and specificity for a combination of five biomarker candidates. We found a combination of five biomarkers for PC. Most of these proteins are known to be related to PC or other gastric cancers, but have never been detected in OF. This study demonstrates the importance of novel OF depletion methodologies for increased protein visibility and highlights the clinical applicability of OF as a diagnostic fluid. Nature Publishing Group UK 2020-12-15 /pmc/articles/PMC7738525/ /pubmed/33319845 http://dx.doi.org/10.1038/s41598-020-78922-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Deutsch, O. Haviv, Y. Krief, G. Keshet, N. Westreich, R. Stemmer, S. M. Zaks, B. Navat, S. P. Yanko, R. Lahav, O. Aframian, D. J. Palmon, A. Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title | Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title_full | Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title_fullStr | Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title_full_unstemmed | Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title_short | Possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
title_sort | possible proteomic biomarkers for the detection of pancreatic cancer in oral fluids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738525/ https://www.ncbi.nlm.nih.gov/pubmed/33319845 http://dx.doi.org/10.1038/s41598-020-78922-x |
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