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In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738553/ https://www.ncbi.nlm.nih.gov/pubmed/33319779 http://dx.doi.org/10.1038/s41467-020-20088-1 |
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author | Gur, Dvir Bain, Emily J. Johnson, Kory R. Aman, Andy J. Pasolli, H. Amalia Flynn, Jessica D. Allen, Michael C. Deheyn, Dimitri D. Lee, Jennifer C. Lippincott-Schwartz, Jennifer Parichy, David M. |
author_facet | Gur, Dvir Bain, Emily J. Johnson, Kory R. Aman, Andy J. Pasolli, H. Amalia Flynn, Jessica D. Allen, Michael C. Deheyn, Dimitri D. Lee, Jennifer C. Lippincott-Schwartz, Jennifer Parichy, David M. |
author_sort | Gur, Dvir |
collection | PubMed |
description | Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called iridophores. It was suggested that the zebrafish’s color pattern arises from a single type of iridophore migrating differentially to stripes and interstripes. However, here we find that iridophores do not migrate between stripes and interstripes but instead differentiate and proliferate in-place, based on their micro-environment. RNA-sequencing analysis further reveals that stripe and interstripe iridophores have different transcriptomic states, while cryogenic-scanning-electron-microscopy and micro-X-ray diffraction identify different crystal-arrays architectures, indicating that stripe and interstripe iridophores are different cell types. Based on these results, we present an alternative model of skin patterning in zebrafish in which distinct iridophore crystallotypes containing specialized, physiologically responsive, organelles arise in stripe and interstripe by in-situ differentiation. |
format | Online Article Text |
id | pubmed-7738553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77385532020-12-28 In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning Gur, Dvir Bain, Emily J. Johnson, Kory R. Aman, Andy J. Pasolli, H. Amalia Flynn, Jessica D. Allen, Michael C. Deheyn, Dimitri D. Lee, Jennifer C. Lippincott-Schwartz, Jennifer Parichy, David M. Nat Commun Article Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called iridophores. It was suggested that the zebrafish’s color pattern arises from a single type of iridophore migrating differentially to stripes and interstripes. However, here we find that iridophores do not migrate between stripes and interstripes but instead differentiate and proliferate in-place, based on their micro-environment. RNA-sequencing analysis further reveals that stripe and interstripe iridophores have different transcriptomic states, while cryogenic-scanning-electron-microscopy and micro-X-ray diffraction identify different crystal-arrays architectures, indicating that stripe and interstripe iridophores are different cell types. Based on these results, we present an alternative model of skin patterning in zebrafish in which distinct iridophore crystallotypes containing specialized, physiologically responsive, organelles arise in stripe and interstripe by in-situ differentiation. Nature Publishing Group UK 2020-12-15 /pmc/articles/PMC7738553/ /pubmed/33319779 http://dx.doi.org/10.1038/s41467-020-20088-1 Text en © The Author(s) 2020, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gur, Dvir Bain, Emily J. Johnson, Kory R. Aman, Andy J. Pasolli, H. Amalia Flynn, Jessica D. Allen, Michael C. Deheyn, Dimitri D. Lee, Jennifer C. Lippincott-Schwartz, Jennifer Parichy, David M. In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title | In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title_full | In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title_fullStr | In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title_full_unstemmed | In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title_short | In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
title_sort | in situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738553/ https://www.ncbi.nlm.nih.gov/pubmed/33319779 http://dx.doi.org/10.1038/s41467-020-20088-1 |
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