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In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning

Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called...

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Autores principales: Gur, Dvir, Bain, Emily J., Johnson, Kory R., Aman, Andy J., Pasolli, H. Amalia, Flynn, Jessica D., Allen, Michael C., Deheyn, Dimitri D., Lee, Jennifer C., Lippincott-Schwartz, Jennifer, Parichy, David M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738553/
https://www.ncbi.nlm.nih.gov/pubmed/33319779
http://dx.doi.org/10.1038/s41467-020-20088-1
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author Gur, Dvir
Bain, Emily J.
Johnson, Kory R.
Aman, Andy J.
Pasolli, H. Amalia
Flynn, Jessica D.
Allen, Michael C.
Deheyn, Dimitri D.
Lee, Jennifer C.
Lippincott-Schwartz, Jennifer
Parichy, David M.
author_facet Gur, Dvir
Bain, Emily J.
Johnson, Kory R.
Aman, Andy J.
Pasolli, H. Amalia
Flynn, Jessica D.
Allen, Michael C.
Deheyn, Dimitri D.
Lee, Jennifer C.
Lippincott-Schwartz, Jennifer
Parichy, David M.
author_sort Gur, Dvir
collection PubMed
description Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called iridophores. It was suggested that the zebrafish’s color pattern arises from a single type of iridophore migrating differentially to stripes and interstripes. However, here we find that iridophores do not migrate between stripes and interstripes but instead differentiate and proliferate in-place, based on their micro-environment. RNA-sequencing analysis further reveals that stripe and interstripe iridophores have different transcriptomic states, while cryogenic-scanning-electron-microscopy and micro-X-ray diffraction identify different crystal-arrays architectures, indicating that stripe and interstripe iridophores are different cell types. Based on these results, we present an alternative model of skin patterning in zebrafish in which distinct iridophore crystallotypes containing specialized, physiologically responsive, organelles arise in stripe and interstripe by in-situ differentiation.
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spelling pubmed-77385532020-12-28 In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning Gur, Dvir Bain, Emily J. Johnson, Kory R. Aman, Andy J. Pasolli, H. Amalia Flynn, Jessica D. Allen, Michael C. Deheyn, Dimitri D. Lee, Jennifer C. Lippincott-Schwartz, Jennifer Parichy, David M. Nat Commun Article Skin color patterns are ubiquitous in nature, impact social behavior, predator avoidance, and protection from ultraviolet irradiation. A leading model system for vertebrate skin patterning is the zebrafish; its alternating blue stripes and yellow interstripes depend on light-reflecting cells called iridophores. It was suggested that the zebrafish’s color pattern arises from a single type of iridophore migrating differentially to stripes and interstripes. However, here we find that iridophores do not migrate between stripes and interstripes but instead differentiate and proliferate in-place, based on their micro-environment. RNA-sequencing analysis further reveals that stripe and interstripe iridophores have different transcriptomic states, while cryogenic-scanning-electron-microscopy and micro-X-ray diffraction identify different crystal-arrays architectures, indicating that stripe and interstripe iridophores are different cell types. Based on these results, we present an alternative model of skin patterning in zebrafish in which distinct iridophore crystallotypes containing specialized, physiologically responsive, organelles arise in stripe and interstripe by in-situ differentiation. Nature Publishing Group UK 2020-12-15 /pmc/articles/PMC7738553/ /pubmed/33319779 http://dx.doi.org/10.1038/s41467-020-20088-1 Text en © The Author(s) 2020, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gur, Dvir
Bain, Emily J.
Johnson, Kory R.
Aman, Andy J.
Pasolli, H. Amalia
Flynn, Jessica D.
Allen, Michael C.
Deheyn, Dimitri D.
Lee, Jennifer C.
Lippincott-Schwartz, Jennifer
Parichy, David M.
In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title_full In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title_fullStr In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title_full_unstemmed In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title_short In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
title_sort in situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738553/
https://www.ncbi.nlm.nih.gov/pubmed/33319779
http://dx.doi.org/10.1038/s41467-020-20088-1
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