Cargando…

Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia

BACKGROUND: Lymphoedema is caused by dysfunction of the lymphatic system resulting in accumulation of high-protein content fluid in the interstitial space. To date, the bacteria associated with wound infections of patients with lower limb lymphoedema in Ethiopia have not been studied. This study ide...

Descripción completa

Detalles Bibliográficos
Autores principales: Nigussie, Dereje, Makonnen, Eyasu, Legesse, Belete Adefris, Fekadu, Abebaw, Davey, Gail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738659/
https://www.ncbi.nlm.nih.gov/pubmed/33247921
http://dx.doi.org/10.1093/trstmh/traa143
_version_ 1783623166437883904
author Nigussie, Dereje
Makonnen, Eyasu
Legesse, Belete Adefris
Fekadu, Abebaw
Davey, Gail
author_facet Nigussie, Dereje
Makonnen, Eyasu
Legesse, Belete Adefris
Fekadu, Abebaw
Davey, Gail
author_sort Nigussie, Dereje
collection PubMed
description BACKGROUND: Lymphoedema is caused by dysfunction of the lymphatic system resulting in accumulation of high-protein content fluid in the interstitial space. To date, the bacteria associated with wound infections of patients with lower limb lymphoedema in Ethiopia have not been studied. This study identified pathogenic bacteria involved in wound infection and assessed antimicrobial susceptibility patterns in patients with lymphoedema in Ethiopia. METHODS: Swab samples were collected from the wounds of patients with lymphoedema and cultured using standard microbiological techniques. Micro-organisms were identified by colony morphology followed by identification and antimicrobial susceptibility testing using the automated VITEK 2 COMPACT Microbial Detection System. RESULTS: Swabs were collected from 103 patients and 84 were culture positive: 44 (52.4%) culture-positive samples showed polymicrobial growth and 40 (47.6%) grew single bacterial isolates. In total, 134 isolates were obtained, of which 26 gram-negative and 12 gram-positive bacterial species were identified. A total of 28/63 (44.4%) gram-negative isolates and 3/57 (5.3%) gram-positive isolates were multiple drug resistant. There was no resistance to ciprofloxacin, moxifloxacin or gentamycin among gram-negative or gram-positive bacteria. CONCLUSION: In this study, many infections were polymicrobial and showed multiple drug resistance. Fluoroquinolones and gentamycin, however, seemed to be effective against bacterial wound infection in this setting.
format Online
Article
Text
id pubmed-7738659
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-77386592020-12-21 Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia Nigussie, Dereje Makonnen, Eyasu Legesse, Belete Adefris Fekadu, Abebaw Davey, Gail Trans R Soc Trop Med Hyg Special Issue BACKGROUND: Lymphoedema is caused by dysfunction of the lymphatic system resulting in accumulation of high-protein content fluid in the interstitial space. To date, the bacteria associated with wound infections of patients with lower limb lymphoedema in Ethiopia have not been studied. This study identified pathogenic bacteria involved in wound infection and assessed antimicrobial susceptibility patterns in patients with lymphoedema in Ethiopia. METHODS: Swab samples were collected from the wounds of patients with lymphoedema and cultured using standard microbiological techniques. Micro-organisms were identified by colony morphology followed by identification and antimicrobial susceptibility testing using the automated VITEK 2 COMPACT Microbial Detection System. RESULTS: Swabs were collected from 103 patients and 84 were culture positive: 44 (52.4%) culture-positive samples showed polymicrobial growth and 40 (47.6%) grew single bacterial isolates. In total, 134 isolates were obtained, of which 26 gram-negative and 12 gram-positive bacterial species were identified. A total of 28/63 (44.4%) gram-negative isolates and 3/57 (5.3%) gram-positive isolates were multiple drug resistant. There was no resistance to ciprofloxacin, moxifloxacin or gentamycin among gram-negative or gram-positive bacteria. CONCLUSION: In this study, many infections were polymicrobial and showed multiple drug resistance. Fluoroquinolones and gentamycin, however, seemed to be effective against bacterial wound infection in this setting. Oxford University Press 2020-11-28 /pmc/articles/PMC7738659/ /pubmed/33247921 http://dx.doi.org/10.1093/trstmh/traa143 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Special Issue
Nigussie, Dereje
Makonnen, Eyasu
Legesse, Belete Adefris
Fekadu, Abebaw
Davey, Gail
Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title_full Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title_fullStr Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title_full_unstemmed Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title_short Antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in East Wollega, Ethiopia
title_sort antimicrobial susceptibility of bacteria isolated from the infected wounds of patients with lymphoedema in east wollega, ethiopia
topic Special Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738659/
https://www.ncbi.nlm.nih.gov/pubmed/33247921
http://dx.doi.org/10.1093/trstmh/traa143
work_keys_str_mv AT nigussiedereje antimicrobialsusceptibilityofbacteriaisolatedfromtheinfectedwoundsofpatientswithlymphoedemaineastwollegaethiopia
AT makonneneyasu antimicrobialsusceptibilityofbacteriaisolatedfromtheinfectedwoundsofpatientswithlymphoedemaineastwollegaethiopia
AT legessebeleteadefris antimicrobialsusceptibilityofbacteriaisolatedfromtheinfectedwoundsofpatientswithlymphoedemaineastwollegaethiopia
AT fekaduabebaw antimicrobialsusceptibilityofbacteriaisolatedfromtheinfectedwoundsofpatientswithlymphoedemaineastwollegaethiopia
AT daveygail antimicrobialsusceptibilityofbacteriaisolatedfromtheinfectedwoundsofpatientswithlymphoedemaineastwollegaethiopia