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Canine atopic dermatitis: Role of luteolin as new natural treatment

BACKGROUND: Luteolin has been demonstrated to possess numerous biological effects. However, the effect of luteolin on LPS (Lipopolysaccharides) stimulation in CPEK cells has not been investigated. HYPOTHESIS/OBJECTIVES: An in vitro model of atopic canine dermatitis was used to identify the antioxida...

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Autores principales: Gugliandolo, Enrico, Palma, Ernesto, Cordaro, Marika, D’Amico, Ramona, Peritore, Alessio Filippo, Licata, Patrizia, Crupi, Rosalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738746/
https://www.ncbi.nlm.nih.gov/pubmed/32741111
http://dx.doi.org/10.1002/vms3.325
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author Gugliandolo, Enrico
Palma, Ernesto
Cordaro, Marika
D’Amico, Ramona
Peritore, Alessio Filippo
Licata, Patrizia
Crupi, Rosalia
author_facet Gugliandolo, Enrico
Palma, Ernesto
Cordaro, Marika
D’Amico, Ramona
Peritore, Alessio Filippo
Licata, Patrizia
Crupi, Rosalia
author_sort Gugliandolo, Enrico
collection PubMed
description BACKGROUND: Luteolin has been demonstrated to possess numerous biological effects. However, the effect of luteolin on LPS (Lipopolysaccharides) stimulation in CPEK cells has not been investigated. HYPOTHESIS/OBJECTIVES: An in vitro model of atopic canine dermatitis was used to identify the antioxidant effect of luteolin as a new treatment that is capable of improving the conditions of veterinary patients. METHODS: CPEK cells were treated with or without luteolin in the presence or absence of LPS. A cell viability assay was performed to test luteolin toxicity and the protective effect of luteolin after LPS stimulation. Additionally, enzyme‐linked immunosorbent assay (ELISA) kits were used to detect the levels of IL‐33, IL‐1β, IL‐6, and IL‐8. RESULTS: Luteolin was capable to significantly decrease levels expression of IL‐33, IL 1β, IL‐6, and IL‐8. CONCLUSIONS AND CLINICAL IMPORTANCE: Luteolin could be a new pharmacological treatment for canine atopic dermatitis.
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spelling pubmed-77387462020-12-18 Canine atopic dermatitis: Role of luteolin as new natural treatment Gugliandolo, Enrico Palma, Ernesto Cordaro, Marika D’Amico, Ramona Peritore, Alessio Filippo Licata, Patrizia Crupi, Rosalia Vet Med Sci Original Articles BACKGROUND: Luteolin has been demonstrated to possess numerous biological effects. However, the effect of luteolin on LPS (Lipopolysaccharides) stimulation in CPEK cells has not been investigated. HYPOTHESIS/OBJECTIVES: An in vitro model of atopic canine dermatitis was used to identify the antioxidant effect of luteolin as a new treatment that is capable of improving the conditions of veterinary patients. METHODS: CPEK cells were treated with or without luteolin in the presence or absence of LPS. A cell viability assay was performed to test luteolin toxicity and the protective effect of luteolin after LPS stimulation. Additionally, enzyme‐linked immunosorbent assay (ELISA) kits were used to detect the levels of IL‐33, IL‐1β, IL‐6, and IL‐8. RESULTS: Luteolin was capable to significantly decrease levels expression of IL‐33, IL 1β, IL‐6, and IL‐8. CONCLUSIONS AND CLINICAL IMPORTANCE: Luteolin could be a new pharmacological treatment for canine atopic dermatitis. John Wiley and Sons Inc. 2020-08-02 /pmc/articles/PMC7738746/ /pubmed/32741111 http://dx.doi.org/10.1002/vms3.325 Text en © 2020 The Authors Veterinary Medicine and Science Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Gugliandolo, Enrico
Palma, Ernesto
Cordaro, Marika
D’Amico, Ramona
Peritore, Alessio Filippo
Licata, Patrizia
Crupi, Rosalia
Canine atopic dermatitis: Role of luteolin as new natural treatment
title Canine atopic dermatitis: Role of luteolin as new natural treatment
title_full Canine atopic dermatitis: Role of luteolin as new natural treatment
title_fullStr Canine atopic dermatitis: Role of luteolin as new natural treatment
title_full_unstemmed Canine atopic dermatitis: Role of luteolin as new natural treatment
title_short Canine atopic dermatitis: Role of luteolin as new natural treatment
title_sort canine atopic dermatitis: role of luteolin as new natural treatment
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738746/
https://www.ncbi.nlm.nih.gov/pubmed/32741111
http://dx.doi.org/10.1002/vms3.325
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