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The expression and clinical significance of TPM4 in hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is known as the fifth most common cancer in the world for its poor prognosis. New diagnostic markers and treatments are urgent to discover. To evaluate the protein expression of Tropomyosin4 (TPM4) and investigate its prognostic value in HCC, we collected 110 patients...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738955/ https://www.ncbi.nlm.nih.gov/pubmed/33390785 http://dx.doi.org/10.7150/ijms.49906 |
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author | Li, Linjing Ye, Tao Zhang, Qingyan Li, Xin Ma, Li Yan, Jing |
author_facet | Li, Linjing Ye, Tao Zhang, Qingyan Li, Xin Ma, Li Yan, Jing |
author_sort | Li, Linjing |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is known as the fifth most common cancer in the world for its poor prognosis. New diagnostic markers and treatments are urgent to discover. To evaluate the protein expression of Tropomyosin4 (TPM4) and investigate its prognostic value in HCC, we collected 110 patients with different degrees of HCC and 10 patients with normal hepatic tissues and performed immunohistochemistry. Western bot was used to evaluate the expression of TPM4 in three HCC cell lines (HepG2, Huh7, SMMC-7721) and normal liver cell line LO2, as well as 7 HCC tissues and 7 normal hepatic tissues. The results of TPM4 staining revealed that TPM4 expression in HCC was higher than that in normal hepatic tissues, which was positive in 51.8% (n=57) and negative in 48.2% (n=53) while in normal hepatic tissues positive staining was in 10% (n=1) and negative staining was in 90% (n=9) (P=0.011). And the expression of TPM4 was related to pT status, grade and stage (P<0.001, P=0.015 and P<0.001, respectively). Western blot results indicated that TPM4 was high expressed in HCC cell line and HCC tissues. In conclusion, we believe that TPM4 can be applied as a diagnostic and prognostic marker to assist the management of HCC. |
format | Online Article Text |
id | pubmed-7738955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-77389552021-01-01 The expression and clinical significance of TPM4 in hepatocellular carcinoma Li, Linjing Ye, Tao Zhang, Qingyan Li, Xin Ma, Li Yan, Jing Int J Med Sci Research Paper Hepatocellular carcinoma (HCC) is known as the fifth most common cancer in the world for its poor prognosis. New diagnostic markers and treatments are urgent to discover. To evaluate the protein expression of Tropomyosin4 (TPM4) and investigate its prognostic value in HCC, we collected 110 patients with different degrees of HCC and 10 patients with normal hepatic tissues and performed immunohistochemistry. Western bot was used to evaluate the expression of TPM4 in three HCC cell lines (HepG2, Huh7, SMMC-7721) and normal liver cell line LO2, as well as 7 HCC tissues and 7 normal hepatic tissues. The results of TPM4 staining revealed that TPM4 expression in HCC was higher than that in normal hepatic tissues, which was positive in 51.8% (n=57) and negative in 48.2% (n=53) while in normal hepatic tissues positive staining was in 10% (n=1) and negative staining was in 90% (n=9) (P=0.011). And the expression of TPM4 was related to pT status, grade and stage (P<0.001, P=0.015 and P<0.001, respectively). Western blot results indicated that TPM4 was high expressed in HCC cell line and HCC tissues. In conclusion, we believe that TPM4 can be applied as a diagnostic and prognostic marker to assist the management of HCC. Ivyspring International Publisher 2021-01-01 /pmc/articles/PMC7738955/ /pubmed/33390785 http://dx.doi.org/10.7150/ijms.49906 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Li, Linjing Ye, Tao Zhang, Qingyan Li, Xin Ma, Li Yan, Jing The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title | The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title_full | The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title_fullStr | The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title_full_unstemmed | The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title_short | The expression and clinical significance of TPM4 in hepatocellular carcinoma |
title_sort | expression and clinical significance of tpm4 in hepatocellular carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738955/ https://www.ncbi.nlm.nih.gov/pubmed/33390785 http://dx.doi.org/10.7150/ijms.49906 |
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