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LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1

Recently, ample evidence indicated that numerous aberrantly expressed long non-coding RNAs (lncRNAs) participated in the development of multiple malignancies. However, the expression and function of lncRNA LOXL1-AS1 in mediating esophageal squamous cell carcinoma (ESCC) carcinogenesis remains largel...

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Autores principales: Li, Hongle, Chu, Jie, Jia, Jinlin, Sheng, Jinxiu, Zhao, Xue, Xing, Yurong, He, Fucheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738998/
https://www.ncbi.nlm.nih.gov/pubmed/33391449
http://dx.doi.org/10.7150/jca.51136
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author Li, Hongle
Chu, Jie
Jia, Jinlin
Sheng, Jinxiu
Zhao, Xue
Xing, Yurong
He, Fucheng
author_facet Li, Hongle
Chu, Jie
Jia, Jinlin
Sheng, Jinxiu
Zhao, Xue
Xing, Yurong
He, Fucheng
author_sort Li, Hongle
collection PubMed
description Recently, ample evidence indicated that numerous aberrantly expressed long non-coding RNAs (lncRNAs) participated in the development of multiple malignancies. However, the expression and function of lncRNA LOXL1-AS1 in mediating esophageal squamous cell carcinoma (ESCC) carcinogenesis remains largely elusive. Here we validated that LOXL1-AS1 was significantly upregulated in ESCC tissues compared with the corresponding adjacent non-neoplastic tissues, and LOXL1-AS1 expression was positively correlated with ESCC patients' lymph node metastasis. Besides, LOXL1-AS1 knockdown impaired ESCC cells proliferation, migration and invasion capabilities in vitro. Furthermore, inhibiting LOXL1-AS1 in ESCC cells increased the percentage of cells at the G1 phase, accompanied by reducing in S phase in contrast to scramble control, and silencing of LOXL1-AS1 evoked ESCC cell apoptosis. From high throughput RNA sequencing (RNA-seq) analysis, we identified that differentially expressed in squamous cell carcinoma 1 (DESC1) was a critical downstream target of LOXL1-AS1. Taken together, we demonstrated the function and mechanism of LOXL1-AS1 in contributing ESCC progression for the first time, and indicated LOXL1-AS1 may be a novel therapeutic biomarker of ESCC.
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spelling pubmed-77389982021-01-01 LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1 Li, Hongle Chu, Jie Jia, Jinlin Sheng, Jinxiu Zhao, Xue Xing, Yurong He, Fucheng J Cancer Research Paper Recently, ample evidence indicated that numerous aberrantly expressed long non-coding RNAs (lncRNAs) participated in the development of multiple malignancies. However, the expression and function of lncRNA LOXL1-AS1 in mediating esophageal squamous cell carcinoma (ESCC) carcinogenesis remains largely elusive. Here we validated that LOXL1-AS1 was significantly upregulated in ESCC tissues compared with the corresponding adjacent non-neoplastic tissues, and LOXL1-AS1 expression was positively correlated with ESCC patients' lymph node metastasis. Besides, LOXL1-AS1 knockdown impaired ESCC cells proliferation, migration and invasion capabilities in vitro. Furthermore, inhibiting LOXL1-AS1 in ESCC cells increased the percentage of cells at the G1 phase, accompanied by reducing in S phase in contrast to scramble control, and silencing of LOXL1-AS1 evoked ESCC cell apoptosis. From high throughput RNA sequencing (RNA-seq) analysis, we identified that differentially expressed in squamous cell carcinoma 1 (DESC1) was a critical downstream target of LOXL1-AS1. Taken together, we demonstrated the function and mechanism of LOXL1-AS1 in contributing ESCC progression for the first time, and indicated LOXL1-AS1 may be a novel therapeutic biomarker of ESCC. Ivyspring International Publisher 2021-01-01 /pmc/articles/PMC7738998/ /pubmed/33391449 http://dx.doi.org/10.7150/jca.51136 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Li, Hongle
Chu, Jie
Jia, Jinlin
Sheng, Jinxiu
Zhao, Xue
Xing, Yurong
He, Fucheng
LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title_full LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title_fullStr LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title_full_unstemmed LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title_short LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1
title_sort lncrna loxl1-as1 promotes esophageal squamous cell carcinoma progression by targeting desc1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738998/
https://www.ncbi.nlm.nih.gov/pubmed/33391449
http://dx.doi.org/10.7150/jca.51136
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