Ultrasensitive, Specific, and Rapid Fluorescence Turn‐On Nitrite Sensor Enabled by Precisely Modulated Fluorophore Binding

The precise regulation of fluorophore binding sites in an organic probe is of great significance toward the design of fluorescent sensing materials with specific functions. In this study, a probe with specific fluorescence properties and nitrite detection ability is designed by precisely modulating benzothiazole binding sites. Only the fluorophore bond at the ortho‐position of the aniline moiety can specifically recognize nitrite, which ensures that the reaction products displays a robust green emission. The unique 2‐(2‐amino‐4‐carboxyphenyl) benzothiazole (ortho‐BT) shows superior nitrite detection performance, including a low detection limit (2.2 fg), rapid detection time (<5 s), and excellent specificity even in the presence of >40 types of strong redox active, colored substances, nitro compounds, and metal ions. Moreover, the probe is highly applicable for the rapid on‐site and semiquantitative measurement of nitrite. The proposed probe design strategy is expected to start a new frontier for the exploration of probe design methodology.