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Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing

Significance: A scene-based adaptive-optics (AO) system is developed and a method for investigating its imaging performance is proposed. The system enables derivation of Strehl ratios from observed images via collaboration with computer simulations. The resultant Strehl ratios are comparable with th...

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Autores principales: Ashida, Yusuke, Honma, Yusuke, Miura, Noriaki, Shibuya, Takatoshi, Kikuchi, Hayao, Tamada, Yosuke, Kamei, Yasuhiro, Matsuda, Atsushi, Hattori, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744043/
https://www.ncbi.nlm.nih.gov/pubmed/33331151
http://dx.doi.org/10.1117/1.JBO.25.12.123707
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author Ashida, Yusuke
Honma, Yusuke
Miura, Noriaki
Shibuya, Takatoshi
Kikuchi, Hayao
Tamada, Yosuke
Kamei, Yasuhiro
Matsuda, Atsushi
Hattori, Masayuki
author_facet Ashida, Yusuke
Honma, Yusuke
Miura, Noriaki
Shibuya, Takatoshi
Kikuchi, Hayao
Tamada, Yosuke
Kamei, Yasuhiro
Matsuda, Atsushi
Hattori, Masayuki
author_sort Ashida, Yusuke
collection PubMed
description Significance: A scene-based adaptive-optics (AO) system is developed and a method for investigating its imaging performance is proposed. The system enables derivation of Strehl ratios from observed images via collaboration with computer simulations. The resultant Strehl ratios are comparable with those of other current AO systems. Aim: For versatile and noninvasive AO microscopy, a scene-based wavefront-sensing technique working on a Shack–Hartmann wavefront sensor is developed in a modal control system. The purpose of the research is to clarify the imaging performance of the AO system via the derivation of Strehl ratios from observed images toward applications in microscopy of living cells and tissues. Approach: Two imaging metrics that can be directly measured from observed images (i.e., an energy concentration ratio and unbiased maximum ratio) are defined and related to the Strehl ratio via computer simulations. Experiments are conducted using artificial targets to measure the imaging metrics, which are then converted to Strehl ratios. Results: The resultant Strehl ratios are [Formula: see text] and 0.5 in the cases of defocus and higher aberrations, respectively. The half-widths at half-maximum of the AO-corrected bead images are favorably comparable to those of on-focus images under simple defocus aberration, and the AO system works both under bright-field illumination and on fluorescent bead images. Conclusions: The proposed scene-based AO system is expected to work with a Strehl ratio of more than 0.5 when applied to high-resolution live imaging of cells and tissues under bright-field and fluorescence microscopies.
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spelling pubmed-77440432020-12-17 Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing Ashida, Yusuke Honma, Yusuke Miura, Noriaki Shibuya, Takatoshi Kikuchi, Hayao Tamada, Yosuke Kamei, Yasuhiro Matsuda, Atsushi Hattori, Masayuki J Biomed Opt Special Series on Biomedical Imaging and Sensing Significance: A scene-based adaptive-optics (AO) system is developed and a method for investigating its imaging performance is proposed. The system enables derivation of Strehl ratios from observed images via collaboration with computer simulations. The resultant Strehl ratios are comparable with those of other current AO systems. Aim: For versatile and noninvasive AO microscopy, a scene-based wavefront-sensing technique working on a Shack–Hartmann wavefront sensor is developed in a modal control system. The purpose of the research is to clarify the imaging performance of the AO system via the derivation of Strehl ratios from observed images toward applications in microscopy of living cells and tissues. Approach: Two imaging metrics that can be directly measured from observed images (i.e., an energy concentration ratio and unbiased maximum ratio) are defined and related to the Strehl ratio via computer simulations. Experiments are conducted using artificial targets to measure the imaging metrics, which are then converted to Strehl ratios. Results: The resultant Strehl ratios are [Formula: see text] and 0.5 in the cases of defocus and higher aberrations, respectively. The half-widths at half-maximum of the AO-corrected bead images are favorably comparable to those of on-focus images under simple defocus aberration, and the AO system works both under bright-field illumination and on fluorescent bead images. Conclusions: The proposed scene-based AO system is expected to work with a Strehl ratio of more than 0.5 when applied to high-resolution live imaging of cells and tissues under bright-field and fluorescence microscopies. Society of Photo-Optical Instrumentation Engineers 2020-12-16 2020-12 /pmc/articles/PMC7744043/ /pubmed/33331151 http://dx.doi.org/10.1117/1.JBO.25.12.123707 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Special Series on Biomedical Imaging and Sensing
Ashida, Yusuke
Honma, Yusuke
Miura, Noriaki
Shibuya, Takatoshi
Kikuchi, Hayao
Tamada, Yosuke
Kamei, Yasuhiro
Matsuda, Atsushi
Hattori, Masayuki
Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title_full Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title_fullStr Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title_full_unstemmed Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title_short Imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
title_sort imaging performance of microscopy adaptive-optics system using scene-based wavefront sensing
topic Special Series on Biomedical Imaging and Sensing
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744043/
https://www.ncbi.nlm.nih.gov/pubmed/33331151
http://dx.doi.org/10.1117/1.JBO.25.12.123707
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