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Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia

BACKGROUND: On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for...

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Autores principales: Dubie, Teshager, Amare, Tsedale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744179/
https://www.ncbi.nlm.nih.gov/pubmed/33376591
http://dx.doi.org/10.1155/2020/8847728
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author Dubie, Teshager
Amare, Tsedale
author_facet Dubie, Teshager
Amare, Tsedale
author_sort Dubie, Teshager
collection PubMed
description BACKGROUND: On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system. RESULTS: The current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% (n = 13) of these cultures exhibited FMDV-induced cytopathic effect (CPE) and the identified serotype was SAT-2 FMD virus. Out of 27 clinical samples tested by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers. Out of 27 samples detected by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers. CONCLUSIONS: Our study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease.
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spelling pubmed-77441792020-12-28 Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia Dubie, Teshager Amare, Tsedale Vet Med Int Research Article BACKGROUND: On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system. RESULTS: The current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% (n = 13) of these cultures exhibited FMDV-induced cytopathic effect (CPE) and the identified serotype was SAT-2 FMD virus. Out of 27 clinical samples tested by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers. Out of 27 samples detected by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers. CONCLUSIONS: Our study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease. Hindawi 2020-12-09 /pmc/articles/PMC7744179/ /pubmed/33376591 http://dx.doi.org/10.1155/2020/8847728 Text en Copyright © 2020 Teshager Dubie and Tsedale Amare. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dubie, Teshager
Amare, Tsedale
Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title_full Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title_fullStr Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title_full_unstemmed Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title_short Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Abaʼala District of Afar Region, Ethiopia
title_sort isolation, serotyping, and molecular detection of bovine fmd virus from outbreak cases in abaʼala district of afar region, ethiopia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744179/
https://www.ncbi.nlm.nih.gov/pubmed/33376591
http://dx.doi.org/10.1155/2020/8847728
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