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Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface

PURPOSE: To investigate the influence of implant surface decontaminated and uncontaminated on osteoblast-like cell adhesion and proliferation MATERIALS AND METHODS: Commercially available implants of different brands and surface characteristics were selected: Biomet 3i® Nanotite (NT) and Osseotite (...

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Autores principales: Balderrama, Ísis de Fátima, Cardoso, Matheus Völz, Stuani, Vitor Toledo, Oliveira, Rodrigo Cardoso, Matos, Adriana Arruda, Greghi, Sebastião Luiz Aguiar, Sant’Ana, Adriana Campos Passanezi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744281/
https://www.ncbi.nlm.nih.gov/pubmed/33330954
http://dx.doi.org/10.1186/s40729-020-00278-8
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author Balderrama, Ísis de Fátima
Cardoso, Matheus Völz
Stuani, Vitor Toledo
Oliveira, Rodrigo Cardoso
Matos, Adriana Arruda
Greghi, Sebastião Luiz Aguiar
Sant’Ana, Adriana Campos Passanezi
author_facet Balderrama, Ísis de Fátima
Cardoso, Matheus Völz
Stuani, Vitor Toledo
Oliveira, Rodrigo Cardoso
Matos, Adriana Arruda
Greghi, Sebastião Luiz Aguiar
Sant’Ana, Adriana Campos Passanezi
author_sort Balderrama, Ísis de Fátima
collection PubMed
description PURPOSE: To investigate the influence of implant surface decontaminated and uncontaminated on osteoblast-like cell adhesion and proliferation MATERIALS AND METHODS: Commercially available implants of different brands and surface characteristics were selected: Biomet 3i® Nanotite (NT) and Osseotite (OT), Straumann® SLActive (SLA), and Neodent® Acqua Drive (ACQ) and Neoporos Drive CM (CM). Physical and chemical properties of the implants were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and wettability analysis (WETT). Implants were previously contaminated with Aggregatibacter actinomycetemcomitans strains; after that, samples were decontaminated by different chemical methods. Decontaminated (test group; n = 15/type of implant) and uncontaminated (control group; n = 5/type of implant) samples were analyzed according to the number of human osteoblastic osteosarcoma cells (Saos-2) adhered on the implant surface after 24 h and 72 h in SEM images. RESULTS: ACQ was found to be highly hydrophilic, and NT was the most hydrophobic implant. Increased variation of Saos-2 cell adhesion and proliferation were observed on all test and control groups. Controversially, at the proliferation analysis in 72 h, CM implant was the only implant that showed no significant difference between test and group (p = 0.2833; Tukey’s multiple comparisons test). NT implants showed the greater value of cell proliferation when compared with all types of implant surface (p = 0.0002; Tukey’s multiple comparisons test). CONCLUSIONS: These findings suggest that decontaminated surfaces were able to impair the counting of osteoblast-like cell adhesion and proliferation.
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spelling pubmed-77442812020-12-28 Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface Balderrama, Ísis de Fátima Cardoso, Matheus Völz Stuani, Vitor Toledo Oliveira, Rodrigo Cardoso Matos, Adriana Arruda Greghi, Sebastião Luiz Aguiar Sant’Ana, Adriana Campos Passanezi Int J Implant Dent Research PURPOSE: To investigate the influence of implant surface decontaminated and uncontaminated on osteoblast-like cell adhesion and proliferation MATERIALS AND METHODS: Commercially available implants of different brands and surface characteristics were selected: Biomet 3i® Nanotite (NT) and Osseotite (OT), Straumann® SLActive (SLA), and Neodent® Acqua Drive (ACQ) and Neoporos Drive CM (CM). Physical and chemical properties of the implants were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and wettability analysis (WETT). Implants were previously contaminated with Aggregatibacter actinomycetemcomitans strains; after that, samples were decontaminated by different chemical methods. Decontaminated (test group; n = 15/type of implant) and uncontaminated (control group; n = 5/type of implant) samples were analyzed according to the number of human osteoblastic osteosarcoma cells (Saos-2) adhered on the implant surface after 24 h and 72 h in SEM images. RESULTS: ACQ was found to be highly hydrophilic, and NT was the most hydrophobic implant. Increased variation of Saos-2 cell adhesion and proliferation were observed on all test and control groups. Controversially, at the proliferation analysis in 72 h, CM implant was the only implant that showed no significant difference between test and group (p = 0.2833; Tukey’s multiple comparisons test). NT implants showed the greater value of cell proliferation when compared with all types of implant surface (p = 0.0002; Tukey’s multiple comparisons test). CONCLUSIONS: These findings suggest that decontaminated surfaces were able to impair the counting of osteoblast-like cell adhesion and proliferation. Springer Berlin Heidelberg 2020-12-17 /pmc/articles/PMC7744281/ /pubmed/33330954 http://dx.doi.org/10.1186/s40729-020-00278-8 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research
Balderrama, Ísis de Fátima
Cardoso, Matheus Völz
Stuani, Vitor Toledo
Oliveira, Rodrigo Cardoso
Matos, Adriana Arruda
Greghi, Sebastião Luiz Aguiar
Sant’Ana, Adriana Campos Passanezi
Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title_full Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title_fullStr Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title_full_unstemmed Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title_short Residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
title_sort residual decontamination chemical agents negatively affect adhesion and proliferation of osteoblast-like cells on implant surface
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744281/
https://www.ncbi.nlm.nih.gov/pubmed/33330954
http://dx.doi.org/10.1186/s40729-020-00278-8
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