Cargando…

A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions

Acinetobacter baumannii is of major clinical importance as the bacterial pathogen often causes hospital acquired infections, further complicated by the high prevalence of antibiotic resistant strains. Aside from natural tolerance to certain antibiotic classes, resistance is often acquired by the exc...

Descripción completa

Detalles Bibliográficos
Autores principales: Loh, Belinda, Chen, Jiayuan, Manohar, Prasanth, Yu, Yunsong, Hua, Xiaoting, Leptihn, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744312/
https://www.ncbi.nlm.nih.gov/pubmed/33343523
http://dx.doi.org/10.3389/fmicb.2020.579802
_version_ 1783624410938212352
author Loh, Belinda
Chen, Jiayuan
Manohar, Prasanth
Yu, Yunsong
Hua, Xiaoting
Leptihn, Sebastian
author_facet Loh, Belinda
Chen, Jiayuan
Manohar, Prasanth
Yu, Yunsong
Hua, Xiaoting
Leptihn, Sebastian
author_sort Loh, Belinda
collection PubMed
description Acinetobacter baumannii is of major clinical importance as the bacterial pathogen often causes hospital acquired infections, further complicated by the high prevalence of antibiotic resistant strains. Aside from natural tolerance to certain antibiotic classes, resistance is often acquired by the exchange of genetic information via conjugation but also by the high natural competence exhibited by A. baumannii. In addition, bacteriophages are able to introduce resistance genes but also toxins and virulence factors via phage mediated transduction. In this work, we analyzed the complete genomes of 177 A. baumannii strains for the occurrence of prophages, and analyzed their taxonomy, size and positions of insertion. Among all the prophages that were detected, Siphoviridae and Myoviridae were the two most commonly found families, while the average genome size was determined to be approximately 4 Mbp. Our data shows the wide variation in the number of prophages in A. baumannii genomes and the prevalence of certain prophages within strains that are most “successful” or potentially beneficial to the host. Our study also revealed that only two specific sites of insertion within the genome of the host bacterium are being used, with few exceptions only. Lastly, we analyzed the existence of genes that are encoded in the prophages, which may confer antimicrobial resistance (AMR). Several phages carry AMR genes, including OXA-23 and NDM-1, illustrating the importance of lysogenic phages in the acquisition of resistance genes.
format Online
Article
Text
id pubmed-7744312
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-77443122020-12-18 A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions Loh, Belinda Chen, Jiayuan Manohar, Prasanth Yu, Yunsong Hua, Xiaoting Leptihn, Sebastian Front Microbiol Microbiology Acinetobacter baumannii is of major clinical importance as the bacterial pathogen often causes hospital acquired infections, further complicated by the high prevalence of antibiotic resistant strains. Aside from natural tolerance to certain antibiotic classes, resistance is often acquired by the exchange of genetic information via conjugation but also by the high natural competence exhibited by A. baumannii. In addition, bacteriophages are able to introduce resistance genes but also toxins and virulence factors via phage mediated transduction. In this work, we analyzed the complete genomes of 177 A. baumannii strains for the occurrence of prophages, and analyzed their taxonomy, size and positions of insertion. Among all the prophages that were detected, Siphoviridae and Myoviridae were the two most commonly found families, while the average genome size was determined to be approximately 4 Mbp. Our data shows the wide variation in the number of prophages in A. baumannii genomes and the prevalence of certain prophages within strains that are most “successful” or potentially beneficial to the host. Our study also revealed that only two specific sites of insertion within the genome of the host bacterium are being used, with few exceptions only. Lastly, we analyzed the existence of genes that are encoded in the prophages, which may confer antimicrobial resistance (AMR). Several phages carry AMR genes, including OXA-23 and NDM-1, illustrating the importance of lysogenic phages in the acquisition of resistance genes. Frontiers Media S.A. 2020-12-03 /pmc/articles/PMC7744312/ /pubmed/33343523 http://dx.doi.org/10.3389/fmicb.2020.579802 Text en Copyright © 2020 Loh, Chen, Manohar, Yu, Hua and Leptihn. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Loh, Belinda
Chen, Jiayuan
Manohar, Prasanth
Yu, Yunsong
Hua, Xiaoting
Leptihn, Sebastian
A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title_full A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title_fullStr A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title_full_unstemmed A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title_short A Biological Inventory of Prophages in A. baumannii Genomes Reveal Distinct Distributions in Classes, Length, and Genomic Positions
title_sort biological inventory of prophages in a. baumannii genomes reveal distinct distributions in classes, length, and genomic positions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744312/
https://www.ncbi.nlm.nih.gov/pubmed/33343523
http://dx.doi.org/10.3389/fmicb.2020.579802
work_keys_str_mv AT lohbelinda abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT chenjiayuan abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT manoharprasanth abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT yuyunsong abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT huaxiaoting abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT leptihnsebastian abiologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT lohbelinda biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT chenjiayuan biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT manoharprasanth biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT yuyunsong biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT huaxiaoting biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions
AT leptihnsebastian biologicalinventoryofprophagesinabaumanniigenomesrevealdistinctdistributionsinclasseslengthandgenomicpositions