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Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum

Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis...

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Autores principales: Xu, Wen, Zhang, Liyong, Goodwin, Paul H., Xia, Mingcong, Zhang, Jie, Wang, Qi, Liang, Juan, Sun, Runhong, Wu, Chao, Yang, Lirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744476/
https://www.ncbi.nlm.nih.gov/pubmed/33343540
http://dx.doi.org/10.3389/fmicb.2020.598285
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author Xu, Wen
Zhang, Liyong
Goodwin, Paul H.
Xia, Mingcong
Zhang, Jie
Wang, Qi
Liang, Juan
Sun, Runhong
Wu, Chao
Yang, Lirong
author_facet Xu, Wen
Zhang, Liyong
Goodwin, Paul H.
Xia, Mingcong
Zhang, Jie
Wang, Qi
Liang, Juan
Sun, Runhong
Wu, Chao
Yang, Lirong
author_sort Xu, Wen
collection PubMed
description Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome annotation, 16S rRNA gene and average nucleotide identities analysis. The whole-genome sequence of strain YB-130 was obtained by PacBio sequencing. 88 putative Carbohydrate-Active Enzymes and 12 gene clusters encoding for secondary metabolites were identified in the YB-130 genome, including one gene cluster for the synthesis of lanthipeptide only found in strain YB-130 genome. In dual cultures, strain YB-130 significantly inhibited the growth of F. graminearum PH-1 and other eight fungal plant pathogens, indicating a broad antifungal activity. Furthermore, strain YB-130 was able to significantly inhibit spore morphology and hyphal development of F. graminearum PH-1. Strain YB-130 also reduced deoxynivalenol production by F. graminearum PH-1 in dual cultures, possibly due to its ability to suppress the expression of tri5, tri3, and tri8 that are required for deoxynivalenol production in F. graminearum. Overall, B. velezensis YB-130 is a promising biological control agent of both F. graminearum infection and mycotoxin production.
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spelling pubmed-77444762020-12-18 Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum Xu, Wen Zhang, Liyong Goodwin, Paul H. Xia, Mingcong Zhang, Jie Wang, Qi Liang, Juan Sun, Runhong Wu, Chao Yang, Lirong Front Microbiol Microbiology Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome annotation, 16S rRNA gene and average nucleotide identities analysis. The whole-genome sequence of strain YB-130 was obtained by PacBio sequencing. 88 putative Carbohydrate-Active Enzymes and 12 gene clusters encoding for secondary metabolites were identified in the YB-130 genome, including one gene cluster for the synthesis of lanthipeptide only found in strain YB-130 genome. In dual cultures, strain YB-130 significantly inhibited the growth of F. graminearum PH-1 and other eight fungal plant pathogens, indicating a broad antifungal activity. Furthermore, strain YB-130 was able to significantly inhibit spore morphology and hyphal development of F. graminearum PH-1. Strain YB-130 also reduced deoxynivalenol production by F. graminearum PH-1 in dual cultures, possibly due to its ability to suppress the expression of tri5, tri3, and tri8 that are required for deoxynivalenol production in F. graminearum. Overall, B. velezensis YB-130 is a promising biological control agent of both F. graminearum infection and mycotoxin production. Frontiers Media S.A. 2020-12-03 /pmc/articles/PMC7744476/ /pubmed/33343540 http://dx.doi.org/10.3389/fmicb.2020.598285 Text en Copyright © 2020 Xu, Zhang, Goodwin, Xia, Zhang, Wang, Liang, Sun, Wu and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xu, Wen
Zhang, Liyong
Goodwin, Paul H.
Xia, Mingcong
Zhang, Jie
Wang, Qi
Liang, Juan
Sun, Runhong
Wu, Chao
Yang, Lirong
Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title_full Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title_fullStr Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title_full_unstemmed Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title_short Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum
title_sort isolation, identification, and complete genome assembly of an endophytic bacillus velezensis yb-130, potential biocontrol agent against fusarium graminearum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744476/
https://www.ncbi.nlm.nih.gov/pubmed/33343540
http://dx.doi.org/10.3389/fmicb.2020.598285
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