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Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering

The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate and pyrrol...

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Autores principales: Manzari-Tavakoli, Asma, Tarasi, Roghayeh, Sedghi, Roya, Moghimi, Ali, Niknejad, Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744540/
https://www.ncbi.nlm.nih.gov/pubmed/33328579
http://dx.doi.org/10.1038/s41598-020-78650-2
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author Manzari-Tavakoli, Asma
Tarasi, Roghayeh
Sedghi, Roya
Moghimi, Ali
Niknejad, Hassan
author_facet Manzari-Tavakoli, Asma
Tarasi, Roghayeh
Sedghi, Roya
Moghimi, Ali
Niknejad, Hassan
author_sort Manzari-Tavakoli, Asma
collection PubMed
description The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl(3) as an oxidant and electrical conductivity of composites were measured by four probe method. In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy–Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold. Physiochemical characterization of nanochitosan/PPy–Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated. Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy–Alg scaffold. Due to the high conductivity, the film with ratio 2:10 (PPy–Alg) was recognized more suitable for fabrication of the final scaffold. Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy–Alg scaffold. Based on the features of the constructed scaffold, Nanochitosan/PPy–Alg scaffold can be a proper candidate for neural tissue engineering.
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spelling pubmed-77445402020-12-17 Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering Manzari-Tavakoli, Asma Tarasi, Roghayeh Sedghi, Roya Moghimi, Ali Niknejad, Hassan Sci Rep Article The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl(3) as an oxidant and electrical conductivity of composites were measured by four probe method. In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy–Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold. Physiochemical characterization of nanochitosan/PPy–Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated. Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy–Alg scaffold. Due to the high conductivity, the film with ratio 2:10 (PPy–Alg) was recognized more suitable for fabrication of the final scaffold. Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy–Alg scaffold. Based on the features of the constructed scaffold, Nanochitosan/PPy–Alg scaffold can be a proper candidate for neural tissue engineering. Nature Publishing Group UK 2020-12-16 /pmc/articles/PMC7744540/ /pubmed/33328579 http://dx.doi.org/10.1038/s41598-020-78650-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Manzari-Tavakoli, Asma
Tarasi, Roghayeh
Sedghi, Roya
Moghimi, Ali
Niknejad, Hassan
Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_full Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_fullStr Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_full_unstemmed Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_short Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_sort fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7744540/
https://www.ncbi.nlm.nih.gov/pubmed/33328579
http://dx.doi.org/10.1038/s41598-020-78650-2
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