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A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge
The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745033/ https://www.ncbi.nlm.nih.gov/pubmed/33328475 http://dx.doi.org/10.1038/s41467-020-20228-7 |
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| author | Yahalom-Ronen, Yfat Tamir, Hadas Melamed, Sharon Politi, Boaz Shifman, Ohad Achdout, Hagit Vitner, Einat B. Israeli, Ofir Milrot, Elad Stein, Dana Cohen-Gihon, Inbar Lazar, Shlomi Gutman, Hila Glinert, Itai Cherry, Lilach Vagima, Yaron Lazar, Shirley Weiss, Shay Ben-Shmuel, Amir Avraham, Roy Puni, Reut Lupu, Edith Bar-David, Elad Sittner, Assa Erez, Noam Zichel, Ran Mamroud, Emanuelle Mazor, Ohad Levy, Haim Laskar, Orly Yitzhaki, Shmuel Shapira, Shmuel C. Zvi, Anat Beth-Din, Adi Paran, Nir Israely, Tomer |
| author_facet | Yahalom-Ronen, Yfat Tamir, Hadas Melamed, Sharon Politi, Boaz Shifman, Ohad Achdout, Hagit Vitner, Einat B. Israeli, Ofir Milrot, Elad Stein, Dana Cohen-Gihon, Inbar Lazar, Shlomi Gutman, Hila Glinert, Itai Cherry, Lilach Vagima, Yaron Lazar, Shirley Weiss, Shay Ben-Shmuel, Amir Avraham, Roy Puni, Reut Lupu, Edith Bar-David, Elad Sittner, Assa Erez, Noam Zichel, Ran Mamroud, Emanuelle Mazor, Ohad Levy, Haim Laskar, Orly Yitzhaki, Shmuel Shapira, Shmuel C. Zvi, Anat Beth-Din, Adi Paran, Nir Israely, Tomer |
| author_sort | Yahalom-Ronen, Yfat |
| collection | PubMed |
| description | The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-∆G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2. |
| format | Online Article Text |
| id | pubmed-7745033 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-77450332020-12-28 A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge Yahalom-Ronen, Yfat Tamir, Hadas Melamed, Sharon Politi, Boaz Shifman, Ohad Achdout, Hagit Vitner, Einat B. Israeli, Ofir Milrot, Elad Stein, Dana Cohen-Gihon, Inbar Lazar, Shlomi Gutman, Hila Glinert, Itai Cherry, Lilach Vagima, Yaron Lazar, Shirley Weiss, Shay Ben-Shmuel, Amir Avraham, Roy Puni, Reut Lupu, Edith Bar-David, Elad Sittner, Assa Erez, Noam Zichel, Ran Mamroud, Emanuelle Mazor, Ohad Levy, Haim Laskar, Orly Yitzhaki, Shmuel Shapira, Shmuel C. Zvi, Anat Beth-Din, Adi Paran, Nir Israely, Tomer Nat Commun Article The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of an efficient and cost-effective vaccine, suitable for mass immunization. Here, we show the development of a replication competent recombinant VSV-∆G-spike vaccine, in which the glycoprotein of VSV is replaced by the spike protein of SARS-CoV-2. In-vitro characterization of this vaccine indicates the expression and presentation of the spike protein on the viral membrane with antigenic similarity to SARS-CoV-2. A golden Syrian hamster in-vivo model for COVID-19 is implemented. We show that a single-dose vaccination results in a rapid and potent induction of SARS-CoV-2 neutralizing antibodies. Importantly, vaccination protects hamsters against SARS-CoV-2 challenge, as demonstrated by the abrogation of body weight loss, and alleviation of the extensive tissue damage and viral loads in lungs and nasal turbinates. Taken together, we suggest the recombinant VSV-∆G-spike as a safe, efficacious and protective vaccine against SARS-CoV-2. Nature Publishing Group UK 2020-12-16 /pmc/articles/PMC7745033/ /pubmed/33328475 http://dx.doi.org/10.1038/s41467-020-20228-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Yahalom-Ronen, Yfat Tamir, Hadas Melamed, Sharon Politi, Boaz Shifman, Ohad Achdout, Hagit Vitner, Einat B. Israeli, Ofir Milrot, Elad Stein, Dana Cohen-Gihon, Inbar Lazar, Shlomi Gutman, Hila Glinert, Itai Cherry, Lilach Vagima, Yaron Lazar, Shirley Weiss, Shay Ben-Shmuel, Amir Avraham, Roy Puni, Reut Lupu, Edith Bar-David, Elad Sittner, Assa Erez, Noam Zichel, Ran Mamroud, Emanuelle Mazor, Ohad Levy, Haim Laskar, Orly Yitzhaki, Shmuel Shapira, Shmuel C. Zvi, Anat Beth-Din, Adi Paran, Nir Israely, Tomer A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title | A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title_full | A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title_fullStr | A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title_full_unstemmed | A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title_short | A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge |
| title_sort | single dose of recombinant vsv-∆g-spike vaccine provides protection against sars-cov-2 challenge |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745033/ https://www.ncbi.nlm.nih.gov/pubmed/33328475 http://dx.doi.org/10.1038/s41467-020-20228-7 |
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