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Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro

OBJECTIVE: Coloring dental biofilm and plaque with a dental disclosing solution is visually effective in dental treatment and oral hygiene education. Despite continuous reports of the risk of the product ingredients, dental disclosing solution are widely used in dentistry. However, the cytotoxic mec...

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Autores principales: Jung, Im‐hee, Yeon, Kyeong Ho, Song, Hwi Rin, Hwang, Young Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745081/
https://www.ncbi.nlm.nih.gov/pubmed/32743893
http://dx.doi.org/10.1002/cre2.321
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author Jung, Im‐hee
Yeon, Kyeong Ho
Song, Hwi Rin
Hwang, Young Sun
author_facet Jung, Im‐hee
Yeon, Kyeong Ho
Song, Hwi Rin
Hwang, Young Sun
author_sort Jung, Im‐hee
collection PubMed
description OBJECTIVE: Coloring dental biofilm and plaque with a dental disclosing solution is visually effective in dental treatment and oral hygiene education. Despite continuous reports of the risk of the product ingredients, dental disclosing solution are widely used in dentistry. However, the cytotoxic mechanism of dental disclosing solution is not known. Here we elucidated the tissue dyeing range and investigated the cytotoxic mechanism of dental disclosing solution. MATERIALS AND METHODS: Gingival epithelial cells and mouse head and neck tissue were stained with dental disclosing solution. Changes in the cell cycle distribution by the dental disclosing solution treatment were analyzed. A deoxynucleotidyl transferase dUTP nick and labeling (TUNEL) assay was performed to examine the apoptotic features of the gingival epithelial cells. RESULTS: Dental disclosing solution stained the chromosome strongly, as well as both the hard and soft tissue of the mouse head and neck. The results of flow cytometric analysis and TUNEL analyses revealed that the cytotoxicity associated with dental disclosing solution was related to the induction of apoptosis. However, the staining of porcine skin by dental disclosing solution was not easily removed, even with a wide range of pH solutions. CONCLUSIONS: These results suggest that dental disclosing solution had strong cytotoxicity and safer alternatives are needed.
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spelling pubmed-77450812020-12-18 Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro Jung, Im‐hee Yeon, Kyeong Ho Song, Hwi Rin Hwang, Young Sun Clin Exp Dent Res Original Articles OBJECTIVE: Coloring dental biofilm and plaque with a dental disclosing solution is visually effective in dental treatment and oral hygiene education. Despite continuous reports of the risk of the product ingredients, dental disclosing solution are widely used in dentistry. However, the cytotoxic mechanism of dental disclosing solution is not known. Here we elucidated the tissue dyeing range and investigated the cytotoxic mechanism of dental disclosing solution. MATERIALS AND METHODS: Gingival epithelial cells and mouse head and neck tissue were stained with dental disclosing solution. Changes in the cell cycle distribution by the dental disclosing solution treatment were analyzed. A deoxynucleotidyl transferase dUTP nick and labeling (TUNEL) assay was performed to examine the apoptotic features of the gingival epithelial cells. RESULTS: Dental disclosing solution stained the chromosome strongly, as well as both the hard and soft tissue of the mouse head and neck. The results of flow cytometric analysis and TUNEL analyses revealed that the cytotoxicity associated with dental disclosing solution was related to the induction of apoptosis. However, the staining of porcine skin by dental disclosing solution was not easily removed, even with a wide range of pH solutions. CONCLUSIONS: These results suggest that dental disclosing solution had strong cytotoxicity and safer alternatives are needed. John Wiley and Sons Inc. 2020-08-02 /pmc/articles/PMC7745081/ /pubmed/32743893 http://dx.doi.org/10.1002/cre2.321 Text en © 2020 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Jung, Im‐hee
Yeon, Kyeong Ho
Song, Hwi Rin
Hwang, Young Sun
Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title_full Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title_fullStr Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title_full_unstemmed Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title_short Cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
title_sort cytotoxicity of dental disclosing solution on gingival epithelial cells in vitro
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745081/
https://www.ncbi.nlm.nih.gov/pubmed/32743893
http://dx.doi.org/10.1002/cre2.321
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