Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus ) evaluated by multiple extraction methods

This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1...

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Detalles Bibliográficos
Autores principales: Moreira, Raulzito Fernandes, Matos, Maria Nágila Carneiro, Alves, João Garcia, do Valle, Roberta Vianna, Eloy, Angela Maria Xavier, Pinto, Tatiana Maria Farias, Machado, Saris Pinto, Costa, Cíntia Renata Rocha, de Lima, José Luiz, Lima, João Paulo Matos Santos, da Cunha, Rodrigo Maranguape Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746222/
https://www.ncbi.nlm.nih.gov/pubmed/33365100
http://dx.doi.org/10.21451/1984-3143-2017-AR966
Descripción
Sumario:This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of detergents increased the resolution of some spots and retained the characteristics of the individual detergents, depending on their concentrations.

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