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Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus ) evaluated by multiple extraction methods

This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1...

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Detalles Bibliográficos
Autores principales: Moreira, Raulzito Fernandes, Matos, Maria Nágila Carneiro, Alves, João Garcia, do Valle, Roberta Vianna, Eloy, Angela Maria Xavier, Pinto, Tatiana Maria Farias, Machado, Saris Pinto, Costa, Cíntia Renata Rocha, de Lima, José Luiz, Lima, João Paulo Matos Santos, da Cunha, Rodrigo Maranguape Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746222/
https://www.ncbi.nlm.nih.gov/pubmed/33365100
http://dx.doi.org/10.21451/1984-3143-2017-AR966