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Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus ) evaluated by multiple extraction methods
This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1...
Autores principales: | Moreira, Raulzito Fernandes, Matos, Maria Nágila Carneiro, Alves, João Garcia, do Valle, Roberta Vianna, Eloy, Angela Maria Xavier, Pinto, Tatiana Maria Farias, Machado, Saris Pinto, Costa, Cíntia Renata Rocha, de Lima, José Luiz, Lima, João Paulo Matos Santos, da Cunha, Rodrigo Maranguape Silva |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746222/ https://www.ncbi.nlm.nih.gov/pubmed/33365100 http://dx.doi.org/10.21451/1984-3143-2017-AR966 |
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