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The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen

Xanthomonas oryzae pv. oryzae (Xoo) is the most infectious pathogen of rice, which causes bacterial leaf blight (BLB) disease. However, the accumulation of chemical or antibiotic resistance of Xoo necessitate the development of its alternative control. In this study, we biologically synthesize three...

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Autores principales: Ogunyemi, Solabomi Olaitan, Zhang, Muchen, Abdallah, Yasmine, Ahmed, Temoor, Qiu, Wen, Ali, Md. Arshad, Yan, Chengqi, Yang, Yong, Chen, Jianping, Li, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746657/
https://www.ncbi.nlm.nih.gov/pubmed/33343527
http://dx.doi.org/10.3389/fmicb.2020.588326
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author Ogunyemi, Solabomi Olaitan
Zhang, Muchen
Abdallah, Yasmine
Ahmed, Temoor
Qiu, Wen
Ali, Md. Arshad
Yan, Chengqi
Yang, Yong
Chen, Jianping
Li, Bin
author_facet Ogunyemi, Solabomi Olaitan
Zhang, Muchen
Abdallah, Yasmine
Ahmed, Temoor
Qiu, Wen
Ali, Md. Arshad
Yan, Chengqi
Yang, Yong
Chen, Jianping
Li, Bin
author_sort Ogunyemi, Solabomi Olaitan
collection PubMed
description Xanthomonas oryzae pv. oryzae (Xoo) is the most infectious pathogen of rice, which causes bacterial leaf blight (BLB) disease. However, the accumulation of chemical or antibiotic resistance of Xoo necessitate the development of its alternative control. In this study, we biologically synthesize three metal oxide nanoparticles (ZnO, MnO(2), and MgO) using rhizophytic bacteria Paenibacillus polymyxa strain Sx3 as reducing agent. The biosynthesis of nanoparticles was confirmed and characterized by using UV-vis spectroscopy, XRD, FTIR, EDS, SEM, and TEM analysis. The UV Vis reflectance of the nanoparticle had peaks at 385, 230, and 230 nm with an average crystallite particle size 62.8, 18.8, and 10.9 nm for ZnO, MnO(2), and MgO, respectively. Biogenic ZnO, MnO(2), and MgO nanoparticles showed substantial significant inhibition effects against Xoo strain GZ 0006 at a concentration of 16.0 μg/ml, for which the antagonized area was 17, 13, and 13 mm and the biofilm formation was decreased by 74.5, 74.4, and 80.2%, respectively. Moreover, the underlining mechanism of nanoparticles was inferred to be in relation to the reactive oxygen species based on their antibacterial efficiency and the deformity in the cell wall phenomenon. Overall, an attractive and eco-friendly biogenic ZnO, MnO(2), and MgO nanoparticles were successfully produced. Altogether, the results suggest that the nanoparticles had an excellent antibacterial efficacy against BLB disease in rice plants, together with the increase in growth parameter and rice biomass. In conclusion, the synthesized nanoparticles could serve as an alternative safe measure in combatting the antibiotic-resistant of Xoo.
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spelling pubmed-77466572020-12-19 The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen Ogunyemi, Solabomi Olaitan Zhang, Muchen Abdallah, Yasmine Ahmed, Temoor Qiu, Wen Ali, Md. Arshad Yan, Chengqi Yang, Yong Chen, Jianping Li, Bin Front Microbiol Microbiology Xanthomonas oryzae pv. oryzae (Xoo) is the most infectious pathogen of rice, which causes bacterial leaf blight (BLB) disease. However, the accumulation of chemical or antibiotic resistance of Xoo necessitate the development of its alternative control. In this study, we biologically synthesize three metal oxide nanoparticles (ZnO, MnO(2), and MgO) using rhizophytic bacteria Paenibacillus polymyxa strain Sx3 as reducing agent. The biosynthesis of nanoparticles was confirmed and characterized by using UV-vis spectroscopy, XRD, FTIR, EDS, SEM, and TEM analysis. The UV Vis reflectance of the nanoparticle had peaks at 385, 230, and 230 nm with an average crystallite particle size 62.8, 18.8, and 10.9 nm for ZnO, MnO(2), and MgO, respectively. Biogenic ZnO, MnO(2), and MgO nanoparticles showed substantial significant inhibition effects against Xoo strain GZ 0006 at a concentration of 16.0 μg/ml, for which the antagonized area was 17, 13, and 13 mm and the biofilm formation was decreased by 74.5, 74.4, and 80.2%, respectively. Moreover, the underlining mechanism of nanoparticles was inferred to be in relation to the reactive oxygen species based on their antibacterial efficiency and the deformity in the cell wall phenomenon. Overall, an attractive and eco-friendly biogenic ZnO, MnO(2), and MgO nanoparticles were successfully produced. Altogether, the results suggest that the nanoparticles had an excellent antibacterial efficacy against BLB disease in rice plants, together with the increase in growth parameter and rice biomass. In conclusion, the synthesized nanoparticles could serve as an alternative safe measure in combatting the antibiotic-resistant of Xoo. Frontiers Media S.A. 2020-12-04 /pmc/articles/PMC7746657/ /pubmed/33343527 http://dx.doi.org/10.3389/fmicb.2020.588326 Text en Copyright © 2020 Ogunyemi, Zhang, Abdallah, Ahmed, Qiu, Ali, Yan, Yang, Chen and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ogunyemi, Solabomi Olaitan
Zhang, Muchen
Abdallah, Yasmine
Ahmed, Temoor
Qiu, Wen
Ali, Md. Arshad
Yan, Chengqi
Yang, Yong
Chen, Jianping
Li, Bin
The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title_full The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title_fullStr The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title_full_unstemmed The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title_short The Bio-Synthesis of Three Metal Oxide Nanoparticles (ZnO, MnO(2), and MgO) and Their Antibacterial Activity Against the Bacterial Leaf Blight Pathogen
title_sort bio-synthesis of three metal oxide nanoparticles (zno, mno(2), and mgo) and their antibacterial activity against the bacterial leaf blight pathogen
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746657/
https://www.ncbi.nlm.nih.gov/pubmed/33343527
http://dx.doi.org/10.3389/fmicb.2020.588326
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