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Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica
Quantitative PCR analysis shows that the virulence plasmid of Salmonella enterica serovar Typhimurium (pSLT) is a low-copy-number plasmid, with 1–2 copies per chromosome. However, fluorescence microscopy observation of pSLT labeled with a lacO fluorescent tag reveals cell-to-cell differences in the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746676/ https://www.ncbi.nlm.nih.gov/pubmed/33343541 http://dx.doi.org/10.3389/fmicb.2020.599931 |
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author | Sánchez-Romero, María A. Mérida-Floriano, Ángela Casadesús, Josep |
author_facet | Sánchez-Romero, María A. Mérida-Floriano, Ángela Casadesús, Josep |
author_sort | Sánchez-Romero, María A. |
collection | PubMed |
description | Quantitative PCR analysis shows that the virulence plasmid of Salmonella enterica serovar Typhimurium (pSLT) is a low-copy-number plasmid, with 1–2 copies per chromosome. However, fluorescence microscopy observation of pSLT labeled with a lacO fluorescent tag reveals cell-to-cell differences in the number of foci, which ranges from 1 to 8. As each focus must correspond to ≥1 plasmid copy, the number of foci can be expected to indicate the minimal number of pSLT copies per cell. A correlation is found between the number of foci and the bacterial cell volume. In contrast, heterogeneity in the number of foci appears to be independent of the cell volume and may have stochastic origin. As a consequence of copy number heterogeneity, expression of a pSLT-bone reporter gene shows high levels of cell-to-cell variation, especially in actively dividing cultures. These observations support the notion that low-copy-number plasmids can be a source of gene expression noise in bacterial populations. |
format | Online Article Text |
id | pubmed-7746676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77466762020-12-19 Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica Sánchez-Romero, María A. Mérida-Floriano, Ángela Casadesús, Josep Front Microbiol Microbiology Quantitative PCR analysis shows that the virulence plasmid of Salmonella enterica serovar Typhimurium (pSLT) is a low-copy-number plasmid, with 1–2 copies per chromosome. However, fluorescence microscopy observation of pSLT labeled with a lacO fluorescent tag reveals cell-to-cell differences in the number of foci, which ranges from 1 to 8. As each focus must correspond to ≥1 plasmid copy, the number of foci can be expected to indicate the minimal number of pSLT copies per cell. A correlation is found between the number of foci and the bacterial cell volume. In contrast, heterogeneity in the number of foci appears to be independent of the cell volume and may have stochastic origin. As a consequence of copy number heterogeneity, expression of a pSLT-bone reporter gene shows high levels of cell-to-cell variation, especially in actively dividing cultures. These observations support the notion that low-copy-number plasmids can be a source of gene expression noise in bacterial populations. Frontiers Media S.A. 2020-12-04 /pmc/articles/PMC7746676/ /pubmed/33343541 http://dx.doi.org/10.3389/fmicb.2020.599931 Text en Copyright © 2020 Sánchez-Romero, Mérida-Floriano and Casadesús. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sánchez-Romero, María A. Mérida-Floriano, Ángela Casadesús, Josep Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title | Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title_full | Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title_fullStr | Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title_full_unstemmed | Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title_short | Copy Number Heterogeneity in the Virulence Plasmid of Salmonella enterica |
title_sort | copy number heterogeneity in the virulence plasmid of salmonella enterica |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746676/ https://www.ncbi.nlm.nih.gov/pubmed/33343541 http://dx.doi.org/10.3389/fmicb.2020.599931 |
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