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Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena

The immediate stress response involves the activation of the monoaminergic neurotransmitter systems including serotonin, dopamine and noradrenaline in particular areas of the fish brain. We chose maraena whitefish as a stress-sensitive salmonid species to investigate the influence of acute and chron...

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Autores principales: Martorell-Ribera, Joan, Venuto, Marzia Tindara, Otten, Winfried, Brunner, Ronald M., Goldammer, Tom, Rebl, Alexander, Gimsa, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746803/
https://www.ncbi.nlm.nih.gov/pubmed/33343287
http://dx.doi.org/10.3389/fnins.2020.591738
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author Martorell-Ribera, Joan
Venuto, Marzia Tindara
Otten, Winfried
Brunner, Ronald M.
Goldammer, Tom
Rebl, Alexander
Gimsa, Ulrike
author_facet Martorell-Ribera, Joan
Venuto, Marzia Tindara
Otten, Winfried
Brunner, Ronald M.
Goldammer, Tom
Rebl, Alexander
Gimsa, Ulrike
author_sort Martorell-Ribera, Joan
collection PubMed
description The immediate stress response involves the activation of the monoaminergic neurotransmitter systems including serotonin, dopamine and noradrenaline in particular areas of the fish brain. We chose maraena whitefish as a stress-sensitive salmonid species to investigate the influence of acute and chronic handling on the neurochemistry of monoamines in the brain. Plasma cortisol was quantified to assess the activation of the stress axis. In addition, we analyzed the expression of 37 genes related to the monoamine system to identify genes that could be used as markers of neurophysiological stress effects. Brain neurochemistry responded to a single handling (1 min netting and chasing) with increased serotonergic activity 3 h post-challenge. This was accompanied by a modulated expression of monoaminergic receptor genes in the hindbrain and a significant increase of plasma cortisol. The initial response was compensated by an increased monoamine synthesis at 24 h post-challenge, combined with the modulated expression of serotonin-receptor genes and plasma cortisol concentrations returning to control levels. After 10 days of repeated handling (1 min per day), we detected a slightly increased noradrenaline synthesis and a down-regulated expression of dopamine-receptor genes without effect on plasma cortisol levels. In conclusion, the changes in serotonergic neurochemistry and selected gene-expression profiles, together with the initial plasma cortisol variation, indicate an acute response and a subsequent recovery phase with signs of habituation after 10 days of daily exposure to handling. Based on the basal expression patterns of particular genes and their significant regulation upon handling conditions, we suggest a group of genes as potential biomarkers that indicate handling stress on the brain monoamine systems.
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spelling pubmed-77468032020-12-19 Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena Martorell-Ribera, Joan Venuto, Marzia Tindara Otten, Winfried Brunner, Ronald M. Goldammer, Tom Rebl, Alexander Gimsa, Ulrike Front Neurosci Neuroscience The immediate stress response involves the activation of the monoaminergic neurotransmitter systems including serotonin, dopamine and noradrenaline in particular areas of the fish brain. We chose maraena whitefish as a stress-sensitive salmonid species to investigate the influence of acute and chronic handling on the neurochemistry of monoamines in the brain. Plasma cortisol was quantified to assess the activation of the stress axis. In addition, we analyzed the expression of 37 genes related to the monoamine system to identify genes that could be used as markers of neurophysiological stress effects. Brain neurochemistry responded to a single handling (1 min netting and chasing) with increased serotonergic activity 3 h post-challenge. This was accompanied by a modulated expression of monoaminergic receptor genes in the hindbrain and a significant increase of plasma cortisol. The initial response was compensated by an increased monoamine synthesis at 24 h post-challenge, combined with the modulated expression of serotonin-receptor genes and plasma cortisol concentrations returning to control levels. After 10 days of repeated handling (1 min per day), we detected a slightly increased noradrenaline synthesis and a down-regulated expression of dopamine-receptor genes without effect on plasma cortisol levels. In conclusion, the changes in serotonergic neurochemistry and selected gene-expression profiles, together with the initial plasma cortisol variation, indicate an acute response and a subsequent recovery phase with signs of habituation after 10 days of daily exposure to handling. Based on the basal expression patterns of particular genes and their significant regulation upon handling conditions, we suggest a group of genes as potential biomarkers that indicate handling stress on the brain monoamine systems. Frontiers Media S.A. 2020-12-04 /pmc/articles/PMC7746803/ /pubmed/33343287 http://dx.doi.org/10.3389/fnins.2020.591738 Text en Copyright © 2020 Martorell-Ribera, Venuto, Otten, Brunner, Goldammer, Rebl and Gimsa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Martorell-Ribera, Joan
Venuto, Marzia Tindara
Otten, Winfried
Brunner, Ronald M.
Goldammer, Tom
Rebl, Alexander
Gimsa, Ulrike
Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title_full Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title_fullStr Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title_full_unstemmed Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title_short Time-Dependent Effects of Acute Handling on the Brain Monoamine System of the Salmonid Coregonus maraena
title_sort time-dependent effects of acute handling on the brain monoamine system of the salmonid coregonus maraena
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746803/
https://www.ncbi.nlm.nih.gov/pubmed/33343287
http://dx.doi.org/10.3389/fnins.2020.591738
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