Cargando…
The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma
RNA methylation accounts for over 60% of all RNA modifications, and N(6)-methyladenosine (m(6)A) is the most common modification on mRNA and lncRNA of human beings. It has been found that m(6)A modification occurs in microRNA, circRNA, rRNA, and tRNA, etc. The m(6)A modification plays an important r...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746824/ https://www.ncbi.nlm.nih.gov/pubmed/33343639 http://dx.doi.org/10.3389/fgene.2020.602485 |
_version_ | 1783624872165900288 |
---|---|
author | Xu, Jiaju Liu, Yuenan Liu, Jingchong Xu, Tianbo Cheng, Gong Shou, Yi Tong, Junwei Liu, Lilong Zhou, Lijie Xiao, Wen Xiong, Zhiyong Yuan, Changfei Chen, Zhixian Liu, Di Yang, Hongmei Liang, Huageng Chen, Ke Zhang, Xiaoping |
author_facet | Xu, Jiaju Liu, Yuenan Liu, Jingchong Xu, Tianbo Cheng, Gong Shou, Yi Tong, Junwei Liu, Lilong Zhou, Lijie Xiao, Wen Xiong, Zhiyong Yuan, Changfei Chen, Zhixian Liu, Di Yang, Hongmei Liang, Huageng Chen, Ke Zhang, Xiaoping |
author_sort | Xu, Jiaju |
collection | PubMed |
description | RNA methylation accounts for over 60% of all RNA modifications, and N(6)-methyladenosine (m(6)A) is the most common modification on mRNA and lncRNA of human beings. It has been found that m(6)A modification occurs in microRNA, circRNA, rRNA, and tRNA, etc. The m(6)A modification plays an important role in regulating gene expression, and the abnormality of its regulatory mechanism refers to many human diseases, including cancers. Pitifully, as it stands there is a serious lack of knowledge of the extent to which the expression and function of m(6)A RNA methylation can influence prostate cancer (PC). Herein, we systematically analyzed the expression levels of 35 m(6)A RNA methylation regulators mentioned in literatures among prostate adenocarcinoma patients in the Cancer Genome Atlas (TCGA), finding that most of them expressed differently between cancer tissues and normal tissues with the significance of p < 0.05. Utilizing consensus clustering, we divided PC patients into two subgroups based on the differentially expressed m(6)A RNA methylation regulators with significantly different clinical outcomes. To appraise the discrepancy in total transcriptome between subgroups, the functional enrichment analysis was conducted for differential signaling pathways and cellular processes. Next, we selected five critical genes by the criteria that the regulators had a significant impact on prognosis of PC patients from TCGA through the last absolute shrinkage and selection operator (LASSO) Cox regression and obtained a risk score by weighted summation for prognosis prediction. The survival analysis curve and receiver operating characteristic (ROC) curve showed that this signature could excellently predict the prognosis of PC patients. The univariate and multivariate Cox regression analyses proved the independent prognostic value of the signature. In summary, our effort revealed the significance of m(6)A RNA methylation regulators in prostate cancer and determined a m(6)A gene expression classifier that well predicted the prognosis of prostate cancer. |
format | Online Article Text |
id | pubmed-7746824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77468242020-12-19 The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma Xu, Jiaju Liu, Yuenan Liu, Jingchong Xu, Tianbo Cheng, Gong Shou, Yi Tong, Junwei Liu, Lilong Zhou, Lijie Xiao, Wen Xiong, Zhiyong Yuan, Changfei Chen, Zhixian Liu, Di Yang, Hongmei Liang, Huageng Chen, Ke Zhang, Xiaoping Front Genet Genetics RNA methylation accounts for over 60% of all RNA modifications, and N(6)-methyladenosine (m(6)A) is the most common modification on mRNA and lncRNA of human beings. It has been found that m(6)A modification occurs in microRNA, circRNA, rRNA, and tRNA, etc. The m(6)A modification plays an important role in regulating gene expression, and the abnormality of its regulatory mechanism refers to many human diseases, including cancers. Pitifully, as it stands there is a serious lack of knowledge of the extent to which the expression and function of m(6)A RNA methylation can influence prostate cancer (PC). Herein, we systematically analyzed the expression levels of 35 m(6)A RNA methylation regulators mentioned in literatures among prostate adenocarcinoma patients in the Cancer Genome Atlas (TCGA), finding that most of them expressed differently between cancer tissues and normal tissues with the significance of p < 0.05. Utilizing consensus clustering, we divided PC patients into two subgroups based on the differentially expressed m(6)A RNA methylation regulators with significantly different clinical outcomes. To appraise the discrepancy in total transcriptome between subgroups, the functional enrichment analysis was conducted for differential signaling pathways and cellular processes. Next, we selected five critical genes by the criteria that the regulators had a significant impact on prognosis of PC patients from TCGA through the last absolute shrinkage and selection operator (LASSO) Cox regression and obtained a risk score by weighted summation for prognosis prediction. The survival analysis curve and receiver operating characteristic (ROC) curve showed that this signature could excellently predict the prognosis of PC patients. The univariate and multivariate Cox regression analyses proved the independent prognostic value of the signature. In summary, our effort revealed the significance of m(6)A RNA methylation regulators in prostate cancer and determined a m(6)A gene expression classifier that well predicted the prognosis of prostate cancer. Frontiers Media S.A. 2020-12-04 /pmc/articles/PMC7746824/ /pubmed/33343639 http://dx.doi.org/10.3389/fgene.2020.602485 Text en Copyright © 2020 Xu, Liu, Liu, Xu, Cheng, Shou, Tong, Liu, Zhou, Xiao, Xiong, Yuan, Chen, Liu, Yang, Liang, Chen and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Xu, Jiaju Liu, Yuenan Liu, Jingchong Xu, Tianbo Cheng, Gong Shou, Yi Tong, Junwei Liu, Lilong Zhou, Lijie Xiao, Wen Xiong, Zhiyong Yuan, Changfei Chen, Zhixian Liu, Di Yang, Hongmei Liang, Huageng Chen, Ke Zhang, Xiaoping The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title | The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title_full | The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title_fullStr | The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title_full_unstemmed | The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title_short | The Identification of Critical m(6)A RNA Methylation Regulators as Malignant Prognosis Factors in Prostate Adenocarcinoma |
title_sort | identification of critical m(6)a rna methylation regulators as malignant prognosis factors in prostate adenocarcinoma |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7746824/ https://www.ncbi.nlm.nih.gov/pubmed/33343639 http://dx.doi.org/10.3389/fgene.2020.602485 |
work_keys_str_mv | AT xujiaju theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liuyuenan theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liujingchong theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xutianbo theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenggong theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT shouyi theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT tongjunwei theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liulilong theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT zhoulijie theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xiaowen theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xiongzhiyong theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT yuanchangfei theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenzhixian theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liudi theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT yanghongmei theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT lianghuageng theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenke theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT zhangxiaoping theidentificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xujiaju identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liuyuenan identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liujingchong identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xutianbo identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenggong identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT shouyi identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT tongjunwei identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liulilong identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT zhoulijie identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xiaowen identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT xiongzhiyong identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT yuanchangfei identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenzhixian identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT liudi identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT yanghongmei identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT lianghuageng identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT chenke identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma AT zhangxiaoping identificationofcriticalm6arnamethylationregulatorsasmalignantprognosisfactorsinprostateadenocarcinoma |