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Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities
In this paper, konjac oligoglucomannan (KOGM) was obtained with a hydrolysis rate of 56.24% by controlling the hydrolysis conditions. KOGM was passed through a 0.2 kDa dialysis bag, a 3 kDa ultrafiltration tube, and a 5 kDa ultrafiltration tube, creating samples with molecular weights of 0.2–3 kDa (...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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De Gruyter
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747514/ https://www.ncbi.nlm.nih.gov/pubmed/33817267 http://dx.doi.org/10.1515/biol-2020-0076 |
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author | Yang, Weidong |
author_facet | Yang, Weidong |
author_sort | Yang, Weidong |
collection | PubMed |
description | In this paper, konjac oligoglucomannan (KOGM) was obtained with a hydrolysis rate of 56.24% by controlling the hydrolysis conditions. KOGM was passed through a 0.2 kDa dialysis bag, a 3 kDa ultrafiltration tube, and a 5 kDa ultrafiltration tube, creating samples with molecular weights of 0.2–3 kDa (IV), 3–5 kDa (III), and >5 kDa (II), respectively. The in vitro antioxidant activities of the KOGM samples were tested by measuring their removal effects on ˙OH, [Image: see text] , and DPPH˙. The in vivo antioxidant activities of the samples were analyzed by measuring their impacts on the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-PX) activity in mice. The results show that the KOGM samples in groups III and IV could effectively remove ˙OH, [Image: see text] , and DPPH˙; the KOGM samples in all three groups could enhance the SOD and GSH-PX activities and reduce the MDA content in the liver tissues of mice; finally, the antioxidant activity of KOGM is negatively correlated with the molecular weight. |
format | Online Article Text |
id | pubmed-7747514 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-77475142021-04-01 Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities Yang, Weidong Open Life Sci Research Article In this paper, konjac oligoglucomannan (KOGM) was obtained with a hydrolysis rate of 56.24% by controlling the hydrolysis conditions. KOGM was passed through a 0.2 kDa dialysis bag, a 3 kDa ultrafiltration tube, and a 5 kDa ultrafiltration tube, creating samples with molecular weights of 0.2–3 kDa (IV), 3–5 kDa (III), and >5 kDa (II), respectively. The in vitro antioxidant activities of the KOGM samples were tested by measuring their removal effects on ˙OH, [Image: see text] , and DPPH˙. The in vivo antioxidant activities of the samples were analyzed by measuring their impacts on the malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, and glutathione peroxidase (GSH-PX) activity in mice. The results show that the KOGM samples in groups III and IV could effectively remove ˙OH, [Image: see text] , and DPPH˙; the KOGM samples in all three groups could enhance the SOD and GSH-PX activities and reduce the MDA content in the liver tissues of mice; finally, the antioxidant activity of KOGM is negatively correlated with the molecular weight. De Gruyter 2020-11-11 /pmc/articles/PMC7747514/ /pubmed/33817267 http://dx.doi.org/10.1515/biol-2020-0076 Text en © 2020 Weidong Yang, published by De Gruyter http://creativecommons.org/licenses/by/4.0 This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Article Yang, Weidong Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title | Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title_full | Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title_fullStr | Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title_full_unstemmed | Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title_short | Preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
title_sort | preparation of konjac oligoglucomannans with different molecular weights and their in vitro and in vivo antioxidant activities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747514/ https://www.ncbi.nlm.nih.gov/pubmed/33817267 http://dx.doi.org/10.1515/biol-2020-0076 |
work_keys_str_mv | AT yangweidong preparationofkonjacoligoglucomannanswithdifferentmolecularweightsandtheirinvitroandinvivoantioxidantactivities |