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A biomimetic engineered bone platform for advanced testing of prosthetic implants

Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advanced testi...

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Autores principales: Sladkova-Faure, Martina, Pujari-Palmer, Michael, Öhman-Mägi, Caroline, López, Alejandro, Wang, Hanbin, Engqvist, Håkan, de Peppo, Giuseppe Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747643/
https://www.ncbi.nlm.nih.gov/pubmed/33335113
http://dx.doi.org/10.1038/s41598-020-78416-w
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author Sladkova-Faure, Martina
Pujari-Palmer, Michael
Öhman-Mägi, Caroline
López, Alejandro
Wang, Hanbin
Engqvist, Håkan
de Peppo, Giuseppe Maria
author_facet Sladkova-Faure, Martina
Pujari-Palmer, Michael
Öhman-Mägi, Caroline
López, Alejandro
Wang, Hanbin
Engqvist, Håkan
de Peppo, Giuseppe Maria
author_sort Sladkova-Faure, Martina
collection PubMed
description Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advanced testing of implants in vitro, and demonstrate the scientific validity and predictive value of this approach using an assortment of complementary evaluation methods. We anchored titanium (Ti) and stainless steel (SS) implants into biomimetic scaffolds, seeded with human induced mesenchymal stem cells, to recapitulate the osseointegration process in vitro. We show distinct patterns of gene expression, matrix deposition, and mineralization in response to the two materials, with Ti implants ultimately resulting in stronger integration strength, as seen in other preclinical and clinical studies. Interestingly, RNAseq analysis reveals that the TGF-beta and the FGF2 pathways are overexpressed in response to Ti implants, while the Wnt, BMP, and IGF pathways are overexpressed in response to SS implants. High-resolution imaging shows significantly increased tissue mineralization and calcium deposition at the tissue-implant interface in response to Ti implants, contributing to a twofold increase in pullout strength compared to SS implants. Our technology creates unprecedented research opportunities towards the design of implants and biomaterials that can be personalized, and exhibit enhanced osseointegration potential, with reduced need for animal testing.
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spelling pubmed-77476432020-12-18 A biomimetic engineered bone platform for advanced testing of prosthetic implants Sladkova-Faure, Martina Pujari-Palmer, Michael Öhman-Mägi, Caroline López, Alejandro Wang, Hanbin Engqvist, Håkan de Peppo, Giuseppe Maria Sci Rep Article Existing methods for testing prosthetic implants suffer from critical limitations, creating an urgent need for new strategies that facilitate research and development of implants with enhanced osseointegration potential. Herein, we describe a novel, biomimetic, human bone platform for advanced testing of implants in vitro, and demonstrate the scientific validity and predictive value of this approach using an assortment of complementary evaluation methods. We anchored titanium (Ti) and stainless steel (SS) implants into biomimetic scaffolds, seeded with human induced mesenchymal stem cells, to recapitulate the osseointegration process in vitro. We show distinct patterns of gene expression, matrix deposition, and mineralization in response to the two materials, with Ti implants ultimately resulting in stronger integration strength, as seen in other preclinical and clinical studies. Interestingly, RNAseq analysis reveals that the TGF-beta and the FGF2 pathways are overexpressed in response to Ti implants, while the Wnt, BMP, and IGF pathways are overexpressed in response to SS implants. High-resolution imaging shows significantly increased tissue mineralization and calcium deposition at the tissue-implant interface in response to Ti implants, contributing to a twofold increase in pullout strength compared to SS implants. Our technology creates unprecedented research opportunities towards the design of implants and biomaterials that can be personalized, and exhibit enhanced osseointegration potential, with reduced need for animal testing. Nature Publishing Group UK 2020-12-17 /pmc/articles/PMC7747643/ /pubmed/33335113 http://dx.doi.org/10.1038/s41598-020-78416-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sladkova-Faure, Martina
Pujari-Palmer, Michael
Öhman-Mägi, Caroline
López, Alejandro
Wang, Hanbin
Engqvist, Håkan
de Peppo, Giuseppe Maria
A biomimetic engineered bone platform for advanced testing of prosthetic implants
title A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_full A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_fullStr A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_full_unstemmed A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_short A biomimetic engineered bone platform for advanced testing of prosthetic implants
title_sort biomimetic engineered bone platform for advanced testing of prosthetic implants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747643/
https://www.ncbi.nlm.nih.gov/pubmed/33335113
http://dx.doi.org/10.1038/s41598-020-78416-w
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