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The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes

Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of...

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Autores principales: Yang, Tianyuan, Feng, Huimin, Zhang, Song, Xiao, Huojun, Hu, Qingdi, Chen, Guang, Xuan, Wei, Moran, Nava, Murphy, Angus, Yu, Ling, Xu, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747981/
https://www.ncbi.nlm.nih.gov/pubmed/33367257
http://dx.doi.org/10.1016/j.xplc.2020.100052
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author Yang, Tianyuan
Feng, Huimin
Zhang, Song
Xiao, Huojun
Hu, Qingdi
Chen, Guang
Xuan, Wei
Moran, Nava
Murphy, Angus
Yu, Ling
Xu, Guohua
author_facet Yang, Tianyuan
Feng, Huimin
Zhang, Song
Xiao, Huojun
Hu, Qingdi
Chen, Guang
Xuan, Wei
Moran, Nava
Murphy, Angus
Yu, Ling
Xu, Guohua
author_sort Yang, Tianyuan
collection PubMed
description Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K(+) supply. Inhibitors of ATP-binding-cassette type-B transporters, NPA and BUM, abolished the OsHAK5-overexpression effect on PAT. The mechanistic basis of these changes included the OsHAK5-mediated decrease of transmembrane potential (depolarization), increase of extracellular pH, and increase of PM-ATPase activity. These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H(+)-ATPase) and regulating ATP-dependent auxin transport. Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations.
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spelling pubmed-77479812020-12-22 The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes Yang, Tianyuan Feng, Huimin Zhang, Song Xiao, Huojun Hu, Qingdi Chen, Guang Xuan, Wei Moran, Nava Murphy, Angus Yu, Ling Xu, Guohua Plant Commun Research Article Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K(+) supply. Inhibitors of ATP-binding-cassette type-B transporters, NPA and BUM, abolished the OsHAK5-overexpression effect on PAT. The mechanistic basis of these changes included the OsHAK5-mediated decrease of transmembrane potential (depolarization), increase of extracellular pH, and increase of PM-ATPase activity. These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H(+)-ATPase) and regulating ATP-dependent auxin transport. Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations. Elsevier 2020-04-29 /pmc/articles/PMC7747981/ /pubmed/33367257 http://dx.doi.org/10.1016/j.xplc.2020.100052 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Yang, Tianyuan
Feng, Huimin
Zhang, Song
Xiao, Huojun
Hu, Qingdi
Chen, Guang
Xuan, Wei
Moran, Nava
Murphy, Angus
Yu, Ling
Xu, Guohua
The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title_full The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title_fullStr The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title_full_unstemmed The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title_short The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
title_sort potassium transporter oshak5 alters rice architecture via atp-dependent transmembrane auxin fluxes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747981/
https://www.ncbi.nlm.nih.gov/pubmed/33367257
http://dx.doi.org/10.1016/j.xplc.2020.100052
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