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The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747981/ https://www.ncbi.nlm.nih.gov/pubmed/33367257 http://dx.doi.org/10.1016/j.xplc.2020.100052 |
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author | Yang, Tianyuan Feng, Huimin Zhang, Song Xiao, Huojun Hu, Qingdi Chen, Guang Xuan, Wei Moran, Nava Murphy, Angus Yu, Ling Xu, Guohua |
author_facet | Yang, Tianyuan Feng, Huimin Zhang, Song Xiao, Huojun Hu, Qingdi Chen, Guang Xuan, Wei Moran, Nava Murphy, Angus Yu, Ling Xu, Guohua |
author_sort | Yang, Tianyuan |
collection | PubMed |
description | Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K(+) supply. Inhibitors of ATP-binding-cassette type-B transporters, NPA and BUM, abolished the OsHAK5-overexpression effect on PAT. The mechanistic basis of these changes included the OsHAK5-mediated decrease of transmembrane potential (depolarization), increase of extracellular pH, and increase of PM-ATPase activity. These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H(+)-ATPase) and regulating ATP-dependent auxin transport. Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations. |
format | Online Article Text |
id | pubmed-7747981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-77479812020-12-22 The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes Yang, Tianyuan Feng, Huimin Zhang, Song Xiao, Huojun Hu, Qingdi Chen, Guang Xuan, Wei Moran, Nava Murphy, Angus Yu, Ling Xu, Guohua Plant Commun Research Article Plant HAK/KUP/KT family members function as plasma membrane (PM) H(+)/K(+) symporters and may modulate chemiosmotically-driven polar auxin transport (PAT). Here, we show that inactivation of OsHAK5, a rice K(+) transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K(+) supply. Inhibitors of ATP-binding-cassette type-B transporters, NPA and BUM, abolished the OsHAK5-overexpression effect on PAT. The mechanistic basis of these changes included the OsHAK5-mediated decrease of transmembrane potential (depolarization), increase of extracellular pH, and increase of PM-ATPase activity. These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H(+)-ATPase) and regulating ATP-dependent auxin transport. Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations. Elsevier 2020-04-29 /pmc/articles/PMC7747981/ /pubmed/33367257 http://dx.doi.org/10.1016/j.xplc.2020.100052 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Yang, Tianyuan Feng, Huimin Zhang, Song Xiao, Huojun Hu, Qingdi Chen, Guang Xuan, Wei Moran, Nava Murphy, Angus Yu, Ling Xu, Guohua The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title | The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title_full | The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title_fullStr | The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title_full_unstemmed | The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title_short | The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes |
title_sort | potassium transporter oshak5 alters rice architecture via atp-dependent transmembrane auxin fluxes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747981/ https://www.ncbi.nlm.nih.gov/pubmed/33367257 http://dx.doi.org/10.1016/j.xplc.2020.100052 |
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