Characterization of complete mitochondrial genome of Nemania diffusa (Xylariaceae, Xylariales) and its phylogenetic analysis

Nemania diffusa is a parasitic fungus of many plant species and causes large economic losses to the forestry industry. In the present study, the complete mitochondrial genome of N. diffusa is first reported. The circular genome is 258,879 bp in length, containing 14 protein coding genes, 2 ribosomal RNA genes, 25 transfer RNA genes. The 258,879 bp long mtDNA of N. diffusa represents one of the largest sequenced fungal mitogenomes. The overall base composition is 34.4% A, 35.9% T, 14.0% G, 15.7% C and the content of GC is 29.7%. Phylogenetic analysis based on concatenated protein coding genes from 24 species in 8 orders was conducted using Bayesian inference (BI) method. Nemania diffusa is clustered in the order Xylariales and is more closely related to Annulohypoxylon stygium of Hypoxylaceae. This work facilitates the future study of molecular biology and evolution of xylariaceous fungi.