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Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis

The complete mitogenome sequence of Blarinella griselda was determined using long PCR. The genome was 16,947 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the he...

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Autores principales: Zhu, Liu, Qiao-Jiang, Yang, Chun-Yu, Xu, Chen, Wang, Qing-Qing, Wang, Wen-Jing, Jiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748876/
https://www.ncbi.nlm.nih.gov/pubmed/33366829
http://dx.doi.org/10.1080/23802359.2020.1715305
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author Zhu, Liu
Qiao-Jiang, Yang
Chun-Yu, Xu
Chen, Wang
Qing-Qing, Wang
Wen-Jing, Jiang
author_facet Zhu, Liu
Qiao-Jiang, Yang
Chun-Yu, Xu
Chen, Wang
Qing-Qing, Wang
Wen-Jing, Jiang
author_sort Zhu, Liu
collection PubMed
description The complete mitogenome sequence of Blarinella griselda was determined using long PCR. The genome was 16,947 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.1%), C (22.6%), T (31.6%) and G (12.7%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Blarinella griselda in Soricidae at the molecular level. The mitochondrial genome would be a significant supplement for the Blarinella griselda genetic background. The three Blarinella species formed a monophyletic group with the high bootstrap value (100%) in all examinations.
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spelling pubmed-77488762020-12-22 Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis Zhu, Liu Qiao-Jiang, Yang Chun-Yu, Xu Chen, Wang Qing-Qing, Wang Wen-Jing, Jiang Mitochondrial DNA B Resour Mitogenome Announcement The complete mitogenome sequence of Blarinella griselda was determined using long PCR. The genome was 16,947 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.1%), C (22.6%), T (31.6%) and G (12.7%). The base compositions present clearly the A–T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of Blarinella griselda in Soricidae at the molecular level. The mitochondrial genome would be a significant supplement for the Blarinella griselda genetic background. The three Blarinella species formed a monophyletic group with the high bootstrap value (100%) in all examinations. Taylor & Francis 2020-02-02 /pmc/articles/PMC7748876/ /pubmed/33366829 http://dx.doi.org/10.1080/23802359.2020.1715305 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mitogenome Announcement
Zhu, Liu
Qiao-Jiang, Yang
Chun-Yu, Xu
Chen, Wang
Qing-Qing, Wang
Wen-Jing, Jiang
Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title_full Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title_fullStr Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title_full_unstemmed Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title_short Sequencing and analysis of the complete mitochondrial genome of Blarinella griselda from China and its phylogenetic analysis
title_sort sequencing and analysis of the complete mitochondrial genome of blarinella griselda from china and its phylogenetic analysis
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748876/
https://www.ncbi.nlm.nih.gov/pubmed/33366829
http://dx.doi.org/10.1080/23802359.2020.1715305
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