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Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats

The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA) against mesenteric ischemia-reperfusion- (I/R-) induced lung and liver injuries and the related mechanisms. Sprague-Dawley (SPD) rats were pretreated for three days with 10 or 40 mg/kg/d, ig of CA, and...

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Autores principales: Almoiliqy, Marwan, Wen, Jin, Qaed, Eskandar, Sun, Yuchao, Lian, Mengqiao, Mousa, Haithm, Al-Azab, Mahmoud, Zaky, Mohamed Y., Chen, Dapeng, Wang, Li, AL-Sharabi, Abdulkarem, Liu, Zhihao, Sun, Pengyuan, Lin, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748914/
https://www.ncbi.nlm.nih.gov/pubmed/33381264
http://dx.doi.org/10.1155/2020/4196548
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author Almoiliqy, Marwan
Wen, Jin
Qaed, Eskandar
Sun, Yuchao
Lian, Mengqiao
Mousa, Haithm
Al-Azab, Mahmoud
Zaky, Mohamed Y.
Chen, Dapeng
Wang, Li
AL-Sharabi, Abdulkarem
Liu, Zhihao
Sun, Pengyuan
Lin, Yuan
author_facet Almoiliqy, Marwan
Wen, Jin
Qaed, Eskandar
Sun, Yuchao
Lian, Mengqiao
Mousa, Haithm
Al-Azab, Mahmoud
Zaky, Mohamed Y.
Chen, Dapeng
Wang, Li
AL-Sharabi, Abdulkarem
Liu, Zhihao
Sun, Pengyuan
Lin, Yuan
author_sort Almoiliqy, Marwan
collection PubMed
description The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA) against mesenteric ischemia-reperfusion- (I/R-) induced lung and liver injuries and the related mechanisms. Sprague-Dawley (SPD) rats were pretreated for three days with 10 or 40 mg/kg/d, ig of CA, and then induced with mesenteric ischemia for 1 h and reperfusion for 2 h. The results indicated that pretreatment with 10 or 40 mg/kg of CA attenuated morphological damage in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly restored the levels of aspartate transaminase (AST) and alanine transaminase (ALT) in mesenteric I/R-injured liver tissues, indicating the improvement of hepatic function. CA also significantly attenuated the inflammation via reducing myeloperoxidase (MOP) activity and downregulating the expression of inflammation-related proteins, including interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (Cox-2), and tumor necrosis factor receptor type-2 (TNFR-2) in both lung and liver tissues of mesenteric I/R-injured rats. Pretreatment with CA significantly downregulated nuclear factor kappa B- (NF-κB-) related protein expressions (NF-κB p65, NF-κB p50, I kappa B alpha (IK-α), and inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ)) in both lung and liver tissues of mesenteric I/R-injured rats. CA also significantly downregulated the protein expression of p53 family members, including caspase-3, caspase-9, Bax, and p53, and restored Bcl-2 in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly reduced TUNEL-apoptotic cells and significantly inhibited p53 and NF-κB p65 nuclear translocation in both lung and liver tissues of mesenteric I/R-injured rats. CA neither induced pulmonary and hepatic histological alterations nor affected the parameters of inflammation and apoptosis in sham rats. We conclude that CA alleviated mesenteric I/R-induced pulmonary and hepatic injuries via attenuating apoptosis and inflammation through inhibition of NF-κB and p53 pathways in rats, suggesting the potential role of CA in remote organ ischemic injury protection.
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spelling pubmed-77489142020-12-29 Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats Almoiliqy, Marwan Wen, Jin Qaed, Eskandar Sun, Yuchao Lian, Mengqiao Mousa, Haithm Al-Azab, Mahmoud Zaky, Mohamed Y. Chen, Dapeng Wang, Li AL-Sharabi, Abdulkarem Liu, Zhihao Sun, Pengyuan Lin, Yuan Oxid Med Cell Longev Research Article The aim of this study was to characterize and reveal the protective effects of cinnamaldehyde (CA) against mesenteric ischemia-reperfusion- (I/R-) induced lung and liver injuries and the related mechanisms. Sprague-Dawley (SPD) rats were pretreated for three days with 10 or 40 mg/kg/d, ig of CA, and then induced with mesenteric ischemia for 1 h and reperfusion for 2 h. The results indicated that pretreatment with 10 or 40 mg/kg of CA attenuated morphological damage in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly restored the levels of aspartate transaminase (AST) and alanine transaminase (ALT) in mesenteric I/R-injured liver tissues, indicating the improvement of hepatic function. CA also significantly attenuated the inflammation via reducing myeloperoxidase (MOP) activity and downregulating the expression of inflammation-related proteins, including interleukin-6 (IL-6), interleukin-1β (IL-1β), cyclooxygenase-2 (Cox-2), and tumor necrosis factor receptor type-2 (TNFR-2) in both lung and liver tissues of mesenteric I/R-injured rats. Pretreatment with CA significantly downregulated nuclear factor kappa B- (NF-κB-) related protein expressions (NF-κB p65, NF-κB p50, I kappa B alpha (IK-α), and inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ)) in both lung and liver tissues of mesenteric I/R-injured rats. CA also significantly downregulated the protein expression of p53 family members, including caspase-3, caspase-9, Bax, and p53, and restored Bcl-2 in both lung and liver tissues of mesenteric I/R-injured rats. CA pretreatment significantly reduced TUNEL-apoptotic cells and significantly inhibited p53 and NF-κB p65 nuclear translocation in both lung and liver tissues of mesenteric I/R-injured rats. CA neither induced pulmonary and hepatic histological alterations nor affected the parameters of inflammation and apoptosis in sham rats. We conclude that CA alleviated mesenteric I/R-induced pulmonary and hepatic injuries via attenuating apoptosis and inflammation through inhibition of NF-κB and p53 pathways in rats, suggesting the potential role of CA in remote organ ischemic injury protection. Hindawi 2020-12-09 /pmc/articles/PMC7748914/ /pubmed/33381264 http://dx.doi.org/10.1155/2020/4196548 Text en Copyright © 2020 Marwan Almoiliqy et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Almoiliqy, Marwan
Wen, Jin
Qaed, Eskandar
Sun, Yuchao
Lian, Mengqiao
Mousa, Haithm
Al-Azab, Mahmoud
Zaky, Mohamed Y.
Chen, Dapeng
Wang, Li
AL-Sharabi, Abdulkarem
Liu, Zhihao
Sun, Pengyuan
Lin, Yuan
Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title_full Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title_fullStr Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title_full_unstemmed Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title_short Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats
title_sort protective effects of cinnamaldehyde against mesenteric ischemia-reperfusion-induced lung and liver injuries in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748914/
https://www.ncbi.nlm.nih.gov/pubmed/33381264
http://dx.doi.org/10.1155/2020/4196548
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