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Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the transient f...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749143/ https://www.ncbi.nlm.nih.gov/pubmed/33339886 http://dx.doi.org/10.1038/s41598-020-79296-w |
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author | Makkay, Andrea M. Louyakis, Artemis S. Ram-Mohan, Nikhil Gophna, Uri Gogarten, J. Peter Papke, R. Thane |
author_facet | Makkay, Andrea M. Louyakis, Artemis S. Ram-Mohan, Nikhil Gophna, Uri Gogarten, J. Peter Papke, R. Thane |
author_sort | Makkay, Andrea M. |
collection | PubMed |
description | Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the transient formation of cytoplasmic bridges between cells and is referred to as mating. This process can result in the exchange of very large fragments of DNA between the participating cells. Genes governing the process of mating, including triggers to initiate mating, mechanisms of cell fusion, and DNA exchange, have yet to be characterized. We used a transcriptomic approach to gain a more detailed knowledge of how mating might transpire. By examining the differential expression of genes expressed in cells harvested from mating conditions on a filter over time and comparing them to those expressed in a shaking culture, we were able to identify genes and pathways potentially associated with mating. These analyses provide new insights into both the mechanisms and barriers of mating in Hfx. volcanii. |
format | Online Article Text |
id | pubmed-7749143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-77491432020-12-22 Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon Makkay, Andrea M. Louyakis, Artemis S. Ram-Mohan, Nikhil Gophna, Uri Gogarten, J. Peter Papke, R. Thane Sci Rep Article Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the transient formation of cytoplasmic bridges between cells and is referred to as mating. This process can result in the exchange of very large fragments of DNA between the participating cells. Genes governing the process of mating, including triggers to initiate mating, mechanisms of cell fusion, and DNA exchange, have yet to be characterized. We used a transcriptomic approach to gain a more detailed knowledge of how mating might transpire. By examining the differential expression of genes expressed in cells harvested from mating conditions on a filter over time and comparing them to those expressed in a shaking culture, we were able to identify genes and pathways potentially associated with mating. These analyses provide new insights into both the mechanisms and barriers of mating in Hfx. volcanii. Nature Publishing Group UK 2020-12-18 /pmc/articles/PMC7749143/ /pubmed/33339886 http://dx.doi.org/10.1038/s41598-020-79296-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Makkay, Andrea M. Louyakis, Artemis S. Ram-Mohan, Nikhil Gophna, Uri Gogarten, J. Peter Papke, R. Thane Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title | Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title_full | Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title_fullStr | Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title_full_unstemmed | Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title_short | Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
title_sort | insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749143/ https://www.ncbi.nlm.nih.gov/pubmed/33339886 http://dx.doi.org/10.1038/s41598-020-79296-w |
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