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Intravenous ketamine for long term anesthesia in rats

Ketamine/xylazine anesthesia has been used primarily for short term procedures in animals, but two prior reports used intravenous ketamine/xylazine for experiments taking many hours. However, there is a discrepancy about the appropriate dose, which is resolved here. Adult Long-Evans rats were used f...

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Autores principales: Linsenmeier, Robert A., Beckmann, Lisa, Dmitriev, Andrey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749388/
https://www.ncbi.nlm.nih.gov/pubmed/33367124
http://dx.doi.org/10.1016/j.heliyon.2020.e05686
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author Linsenmeier, Robert A.
Beckmann, Lisa
Dmitriev, Andrey V.
author_facet Linsenmeier, Robert A.
Beckmann, Lisa
Dmitriev, Andrey V.
author_sort Linsenmeier, Robert A.
collection PubMed
description Ketamine/xylazine anesthesia has been used primarily for short term procedures in animals, but two prior reports used intravenous ketamine/xylazine for experiments taking many hours. However, there is a discrepancy about the appropriate dose, which is resolved here. Adult Long-Evans rats were used for recording from the retina. Doses of Ketamine/xylazine were adjusted to minimize anesthetic in terminal experiments lasting 10 h. An allometric relation was fitted to the resulting data on doses as a function of body weight, and compared to prior work. The allometric relationship between the continuously infused specific dose and weight was: dose = 9.13 (weight)(−1.213) (r(2) = 0.73), where dose is in mg-kg(−1)-hr(−1) and rat weight is in kg. The dose of xylazine was 3.3% of the ketamine dose. No attempt was made to explore different relative doses of xylazine and ketamine. Prior work is consistent with this relationship, showing that the earlier discrepancy resulted from using rats of different sizes. Ketamine at the doses used here still depressed the electroretinogram relative to historical controls using urethane. We conclude that intravenous ketamine dosing in rats should not use the same mg-kg(−1)-hr(−1) dose for all rats, but take into account the strong allometric relationship between dose and rat weight. There is an advantage in using smaller doses in order to prevent unnecessary depression of neural responses.
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spelling pubmed-77493882020-12-22 Intravenous ketamine for long term anesthesia in rats Linsenmeier, Robert A. Beckmann, Lisa Dmitriev, Andrey V. Heliyon Research Article Ketamine/xylazine anesthesia has been used primarily for short term procedures in animals, but two prior reports used intravenous ketamine/xylazine for experiments taking many hours. However, there is a discrepancy about the appropriate dose, which is resolved here. Adult Long-Evans rats were used for recording from the retina. Doses of Ketamine/xylazine were adjusted to minimize anesthetic in terminal experiments lasting 10 h. An allometric relation was fitted to the resulting data on doses as a function of body weight, and compared to prior work. The allometric relationship between the continuously infused specific dose and weight was: dose = 9.13 (weight)(−1.213) (r(2) = 0.73), where dose is in mg-kg(−1)-hr(−1) and rat weight is in kg. The dose of xylazine was 3.3% of the ketamine dose. No attempt was made to explore different relative doses of xylazine and ketamine. Prior work is consistent with this relationship, showing that the earlier discrepancy resulted from using rats of different sizes. Ketamine at the doses used here still depressed the electroretinogram relative to historical controls using urethane. We conclude that intravenous ketamine dosing in rats should not use the same mg-kg(−1)-hr(−1) dose for all rats, but take into account the strong allometric relationship between dose and rat weight. There is an advantage in using smaller doses in order to prevent unnecessary depression of neural responses. Elsevier 2020-12-14 /pmc/articles/PMC7749388/ /pubmed/33367124 http://dx.doi.org/10.1016/j.heliyon.2020.e05686 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Linsenmeier, Robert A.
Beckmann, Lisa
Dmitriev, Andrey V.
Intravenous ketamine for long term anesthesia in rats
title Intravenous ketamine for long term anesthesia in rats
title_full Intravenous ketamine for long term anesthesia in rats
title_fullStr Intravenous ketamine for long term anesthesia in rats
title_full_unstemmed Intravenous ketamine for long term anesthesia in rats
title_short Intravenous ketamine for long term anesthesia in rats
title_sort intravenous ketamine for long term anesthesia in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7749388/
https://www.ncbi.nlm.nih.gov/pubmed/33367124
http://dx.doi.org/10.1016/j.heliyon.2020.e05686
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