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Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India

BACKGROUND AND AIM: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months. The study was undertaken to detect the pathogenic Leptospira...

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Autores principales: Rohilla, Preeti, Khurana, Rajesh, Kumar, Aman, Batra, Kanisht, Gupta, Renu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750226/
https://www.ncbi.nlm.nih.gov/pubmed/33363334
http://dx.doi.org/10.14202/vetworld.2020.2411-2415
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author Rohilla, Preeti
Khurana, Rajesh
Kumar, Aman
Batra, Kanisht
Gupta, Renu
author_facet Rohilla, Preeti
Khurana, Rajesh
Kumar, Aman
Batra, Kanisht
Gupta, Renu
author_sort Rohilla, Preeti
collection PubMed
description BACKGROUND AND AIM: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months. The study was undertaken to detect the pathogenic Leptospira in healthy dogs’ urinary shedding by real-time polymerase chain reaction (qPCR). MATERIALS AND METHODS: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. To detect the pathogenic Leptospira organisms in dogs’ urinary shedding, 239 urine samples were collected from healthy dogs from April 2018 to March 2019 from different areas of Haryana. All the urine samples were processed for DNA extraction and qPCR technique was used to detect the presence of Leptospira. RESULTS: Out of 239 urine samples of dogs, none of the samples resulted in the detection of DNA of pathogenic Leptospira organisms. CONCLUSION: The present study indicated low risk of transmission of Leptospira organisms from dogs’ urine to human beings in Haryana.
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spelling pubmed-77502262020-12-23 Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India Rohilla, Preeti Khurana, Rajesh Kumar, Aman Batra, Kanisht Gupta, Renu Vet World Research Article BACKGROUND AND AIM: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months. The study was undertaken to detect the pathogenic Leptospira in healthy dogs’ urinary shedding by real-time polymerase chain reaction (qPCR). MATERIALS AND METHODS: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. To detect the pathogenic Leptospira organisms in dogs’ urinary shedding, 239 urine samples were collected from healthy dogs from April 2018 to March 2019 from different areas of Haryana. All the urine samples were processed for DNA extraction and qPCR technique was used to detect the presence of Leptospira. RESULTS: Out of 239 urine samples of dogs, none of the samples resulted in the detection of DNA of pathogenic Leptospira organisms. CONCLUSION: The present study indicated low risk of transmission of Leptospira organisms from dogs’ urine to human beings in Haryana. Veterinary World 2020-11 2020-11-12 /pmc/articles/PMC7750226/ /pubmed/33363334 http://dx.doi.org/10.14202/vetworld.2020.2411-2415 Text en Copyright: © Rohilla, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rohilla, Preeti
Khurana, Rajesh
Kumar, Aman
Batra, Kanisht
Gupta, Renu
Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title_full Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title_fullStr Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title_full_unstemmed Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title_short Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India
title_sort detection of leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in haryana, india
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750226/
https://www.ncbi.nlm.nih.gov/pubmed/33363334
http://dx.doi.org/10.14202/vetworld.2020.2411-2415
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