Cargando…
Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene
BACKGROUND AND AIM: Indonesian cuscuses are now becoming scarce because of the reduction of habitat and poaching. Further, molecular characterization of Indonesian cuscuses is still very lacking. This study aimed to determine genetic markers and phylogenetic relationships of Indonesian cuscuses base...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750240/ https://www.ncbi.nlm.nih.gov/pubmed/33363321 http://dx.doi.org/10.14202/vetworld.2020.2319-2325 |
_version_ | 1783625453985071104 |
---|---|
author | Widayanti, Rini Pradana, Richo Apriladi Bagas Kunda, Rony Marsyal Pakpahan, Suhendra |
author_facet | Widayanti, Rini Pradana, Richo Apriladi Bagas Kunda, Rony Marsyal Pakpahan, Suhendra |
author_sort | Widayanti, Rini |
collection | PubMed |
description | BACKGROUND AND AIM: Indonesian cuscuses are now becoming scarce because of the reduction of habitat and poaching. Further, molecular characterization of Indonesian cuscuses is still very lacking. This study aimed to determine genetic markers and phylogenetic relationships of Indonesian cuscuses based on 16S rRNA gene sequences. MATERIALS AND METHODS: This study used 21 cuscuses caught from two provinces and 16 islands: 13 from Maluku and eight from Papua. Cuscus samples were taken by biopsy following ethics guidelines for animals. The genome isolation was done using gSYNC DNA Mini Kit (Geneaid Biotech Ltd., Taiwan). The 16S rRNA gene was amplified by primers (16SKUSAF and 16SKUSAR), and the polymerase chain reaction product obtained was 1875 base pair (bp). The analysis of genetic characterization and the phylogenetic relationship was performed usingMEGA version X software (https://www.megasoftware.net/). RESULTS: 16S rRNA gene sequencing attained 1598 bp for all samples. Based on the 16S rRNA nucleotide sequences, cuscuses from Papua and Maluku belong to the genus Phalanger and Spilocuscus. Phalanger spp. and Spilocuscus spp. from Papua can be distinguished from Phalanger and Spilocuscus from Maluku, except Spilocuscus from Ternate has a very close relationship with cuscus from Sentani, Papua. CONCLUSION: Indonesian cuscuses were derived into two clades based on 16S rRNA gene sequence, one group to genus Phalanger and another group to Spilocuscus. |
format | Online Article Text |
id | pubmed-7750240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-77502402020-12-23 Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene Widayanti, Rini Pradana, Richo Apriladi Bagas Kunda, Rony Marsyal Pakpahan, Suhendra Vet World Research Article BACKGROUND AND AIM: Indonesian cuscuses are now becoming scarce because of the reduction of habitat and poaching. Further, molecular characterization of Indonesian cuscuses is still very lacking. This study aimed to determine genetic markers and phylogenetic relationships of Indonesian cuscuses based on 16S rRNA gene sequences. MATERIALS AND METHODS: This study used 21 cuscuses caught from two provinces and 16 islands: 13 from Maluku and eight from Papua. Cuscus samples were taken by biopsy following ethics guidelines for animals. The genome isolation was done using gSYNC DNA Mini Kit (Geneaid Biotech Ltd., Taiwan). The 16S rRNA gene was amplified by primers (16SKUSAF and 16SKUSAR), and the polymerase chain reaction product obtained was 1875 base pair (bp). The analysis of genetic characterization and the phylogenetic relationship was performed usingMEGA version X software (https://www.megasoftware.net/). RESULTS: 16S rRNA gene sequencing attained 1598 bp for all samples. Based on the 16S rRNA nucleotide sequences, cuscuses from Papua and Maluku belong to the genus Phalanger and Spilocuscus. Phalanger spp. and Spilocuscus spp. from Papua can be distinguished from Phalanger and Spilocuscus from Maluku, except Spilocuscus from Ternate has a very close relationship with cuscus from Sentani, Papua. CONCLUSION: Indonesian cuscuses were derived into two clades based on 16S rRNA gene sequence, one group to genus Phalanger and another group to Spilocuscus. Veterinary World 2020-11 2020-11-04 /pmc/articles/PMC7750240/ /pubmed/33363321 http://dx.doi.org/10.14202/vetworld.2020.2319-2325 Text en Copyright: © Widayanti, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Widayanti, Rini Pradana, Richo Apriladi Bagas Kunda, Rony Marsyal Pakpahan, Suhendra Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title | Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title_full | Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title_fullStr | Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title_full_unstemmed | Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title_short | Genetic characterization and phylogenetic study of Indonesian cuscuses from Maluku and Papua Island based on 16S rRNA gene |
title_sort | genetic characterization and phylogenetic study of indonesian cuscuses from maluku and papua island based on 16s rrna gene |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750240/ https://www.ncbi.nlm.nih.gov/pubmed/33363321 http://dx.doi.org/10.14202/vetworld.2020.2319-2325 |
work_keys_str_mv | AT widayantirini geneticcharacterizationandphylogeneticstudyofindonesiancuscusesfrommalukuandpapuaislandbasedon16srrnagene AT pradanarichoapriladibagas geneticcharacterizationandphylogeneticstudyofindonesiancuscusesfrommalukuandpapuaislandbasedon16srrnagene AT kundaronymarsyal geneticcharacterizationandphylogeneticstudyofindonesiancuscusesfrommalukuandpapuaislandbasedon16srrnagene AT pakpahansuhendra geneticcharacterizationandphylogeneticstudyofindonesiancuscusesfrommalukuandpapuaislandbasedon16srrnagene |