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Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Equine Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750643/ https://www.ncbi.nlm.nih.gov/pubmed/33376444 http://dx.doi.org/10.1294/jes.31.85 |
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author | SATO, Wataru SUKMAWINATA, Eddy UEMURA, Ryoko KANDA, Takuya KUSANO, Kanichi KAMBAYASHI, Yoshinori SATO, Takashi ISHIKAWA, Yuhiro TOYA, Ryohei SUEYOSHI, Masuo |
author_facet | SATO, Wataru SUKMAWINATA, Eddy UEMURA, Ryoko KANDA, Takuya KUSANO, Kanichi KAMBAYASHI, Yoshinori SATO, Takashi ISHIKAWA, Yuhiro TOYA, Ryohei SUEYOSHI, Masuo |
author_sort | SATO, Wataru |
collection | PubMed |
description | In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association training centers. E. coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). A total of 417 E. coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. The 417 E. coli isolates were phylogenetically grouped using a multiplex polymerase chain reaction. The highest proportion of resistance was observed for streptomycin (30.9%, 129/417) followed by ampicillin (19.4%, 81/417), trimethoprim (15.8%, 66/417), tetracycline (8.4%, 35/417), chloramphenicol (2.6%, 11/417), kanamycin (1.2%, 5/417), nalidixic acid (0.5%, 2/417), cefazolin (0.2%, 1/417), colistin (0.2%, 1/417), and gentamycin (0%). Multidrug-resistant (MDR) E. coli was detected in 7.9% (33/417) of isolates. The proportions of resistance against ampicillin, streptomycin, kanamycin, and chloramphenicol and of multidrug-resistant phenotypes in E. coli belonging to phylogenetic group B2 were significantly higher than those of other groups. This study clarified the distribution of antimicrobial-resistant (AMR) E. coli in Japanese racehorses. A continuous monitoring program for antimicrobial resistance is required to control the spread of AMR bacteria in racehorses. |
format | Online Article Text |
id | pubmed-7750643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Japanese Society of Equine Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-77506432020-12-28 Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan SATO, Wataru SUKMAWINATA, Eddy UEMURA, Ryoko KANDA, Takuya KUSANO, Kanichi KAMBAYASHI, Yoshinori SATO, Takashi ISHIKAWA, Yuhiro TOYA, Ryohei SUEYOSHI, Masuo J Equine Sci Full Paper In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association training centers. E. coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). A total of 417 E. coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. The 417 E. coli isolates were phylogenetically grouped using a multiplex polymerase chain reaction. The highest proportion of resistance was observed for streptomycin (30.9%, 129/417) followed by ampicillin (19.4%, 81/417), trimethoprim (15.8%, 66/417), tetracycline (8.4%, 35/417), chloramphenicol (2.6%, 11/417), kanamycin (1.2%, 5/417), nalidixic acid (0.5%, 2/417), cefazolin (0.2%, 1/417), colistin (0.2%, 1/417), and gentamycin (0%). Multidrug-resistant (MDR) E. coli was detected in 7.9% (33/417) of isolates. The proportions of resistance against ampicillin, streptomycin, kanamycin, and chloramphenicol and of multidrug-resistant phenotypes in E. coli belonging to phylogenetic group B2 were significantly higher than those of other groups. This study clarified the distribution of antimicrobial-resistant (AMR) E. coli in Japanese racehorses. A continuous monitoring program for antimicrobial resistance is required to control the spread of AMR bacteria in racehorses. The Japanese Society of Equine Science 2020-12-18 2020-12 /pmc/articles/PMC7750643/ /pubmed/33376444 http://dx.doi.org/10.1294/jes.31.85 Text en ©2020 The Japanese Society of Equine Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Full Paper SATO, Wataru SUKMAWINATA, Eddy UEMURA, Ryoko KANDA, Takuya KUSANO, Kanichi KAMBAYASHI, Yoshinori SATO, Takashi ISHIKAWA, Yuhiro TOYA, Ryohei SUEYOSHI, Masuo Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan |
title | Antimicrobial resistance profiles and phylogenetic groups of
Escherichia coli isolated from healthy Thoroughbred racehorses in
Japan |
title_full | Antimicrobial resistance profiles and phylogenetic groups of
Escherichia coli isolated from healthy Thoroughbred racehorses in
Japan |
title_fullStr | Antimicrobial resistance profiles and phylogenetic groups of
Escherichia coli isolated from healthy Thoroughbred racehorses in
Japan |
title_full_unstemmed | Antimicrobial resistance profiles and phylogenetic groups of
Escherichia coli isolated from healthy Thoroughbred racehorses in
Japan |
title_short | Antimicrobial resistance profiles and phylogenetic groups of
Escherichia coli isolated from healthy Thoroughbred racehorses in
Japan |
title_sort | antimicrobial resistance profiles and phylogenetic groups of
escherichia coli isolated from healthy thoroughbred racehorses in
japan |
topic | Full Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750643/ https://www.ncbi.nlm.nih.gov/pubmed/33376444 http://dx.doi.org/10.1294/jes.31.85 |
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