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Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan

In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association...

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Autores principales: SATO, Wataru, SUKMAWINATA, Eddy, UEMURA, Ryoko, KANDA, Takuya, KUSANO, Kanichi, KAMBAYASHI, Yoshinori, SATO, Takashi, ISHIKAWA, Yuhiro, TOYA, Ryohei, SUEYOSHI, Masuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Equine Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750643/
https://www.ncbi.nlm.nih.gov/pubmed/33376444
http://dx.doi.org/10.1294/jes.31.85
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author SATO, Wataru
SUKMAWINATA, Eddy
UEMURA, Ryoko
KANDA, Takuya
KUSANO, Kanichi
KAMBAYASHI, Yoshinori
SATO, Takashi
ISHIKAWA, Yuhiro
TOYA, Ryohei
SUEYOSHI, Masuo
author_facet SATO, Wataru
SUKMAWINATA, Eddy
UEMURA, Ryoko
KANDA, Takuya
KUSANO, Kanichi
KAMBAYASHI, Yoshinori
SATO, Takashi
ISHIKAWA, Yuhiro
TOYA, Ryohei
SUEYOSHI, Masuo
author_sort SATO, Wataru
collection PubMed
description In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association training centers. E. coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). A total of 417 E. coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. The 417 E. coli isolates were phylogenetically grouped using a multiplex polymerase chain reaction. The highest proportion of resistance was observed for streptomycin (30.9%, 129/417) followed by ampicillin (19.4%, 81/417), trimethoprim (15.8%, 66/417), tetracycline (8.4%, 35/417), chloramphenicol (2.6%, 11/417), kanamycin (1.2%, 5/417), nalidixic acid (0.5%, 2/417), cefazolin (0.2%, 1/417), colistin (0.2%, 1/417), and gentamycin (0%). Multidrug-resistant (MDR) E. coli was detected in 7.9% (33/417) of isolates. The proportions of resistance against ampicillin, streptomycin, kanamycin, and chloramphenicol and of multidrug-resistant phenotypes in E. coli belonging to phylogenetic group B2 were significantly higher than those of other groups. This study clarified the distribution of antimicrobial-resistant (AMR) E. coli in Japanese racehorses. A continuous monitoring program for antimicrobial resistance is required to control the spread of AMR bacteria in racehorses.
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spelling pubmed-77506432020-12-28 Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan SATO, Wataru SUKMAWINATA, Eddy UEMURA, Ryoko KANDA, Takuya KUSANO, Kanichi KAMBAYASHI, Yoshinori SATO, Takashi ISHIKAWA, Yuhiro TOYA, Ryohei SUEYOSHI, Masuo J Equine Sci Full Paper In this study, we investigated the occurrence of antimicrobial resistance in commensal Escherichia coli isolated from healthy Thoroughbred (TB) racehorses in Japan. A total of 212 fecal samples were individually collected from TB racehorses from March 2017 to August 2018 at Japan Racing Association training centers. E. coli was isolated by using selective agar media, deoxycholate-hydrogen sulfide-lactose (DHL) and eosin methylene blue (EMB). A total of 417 E. coli isolates were examined against 10 antimicrobial agents by using the broth microdilution method. The 417 E. coli isolates were phylogenetically grouped using a multiplex polymerase chain reaction. The highest proportion of resistance was observed for streptomycin (30.9%, 129/417) followed by ampicillin (19.4%, 81/417), trimethoprim (15.8%, 66/417), tetracycline (8.4%, 35/417), chloramphenicol (2.6%, 11/417), kanamycin (1.2%, 5/417), nalidixic acid (0.5%, 2/417), cefazolin (0.2%, 1/417), colistin (0.2%, 1/417), and gentamycin (0%). Multidrug-resistant (MDR) E. coli was detected in 7.9% (33/417) of isolates. The proportions of resistance against ampicillin, streptomycin, kanamycin, and chloramphenicol and of multidrug-resistant phenotypes in E. coli belonging to phylogenetic group B2 were significantly higher than those of other groups. This study clarified the distribution of antimicrobial-resistant (AMR) E. coli in Japanese racehorses. A continuous monitoring program for antimicrobial resistance is required to control the spread of AMR bacteria in racehorses. The Japanese Society of Equine Science 2020-12-18 2020-12 /pmc/articles/PMC7750643/ /pubmed/33376444 http://dx.doi.org/10.1294/jes.31.85 Text en ©2020 The Japanese Society of Equine Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Full Paper
SATO, Wataru
SUKMAWINATA, Eddy
UEMURA, Ryoko
KANDA, Takuya
KUSANO, Kanichi
KAMBAYASHI, Yoshinori
SATO, Takashi
ISHIKAWA, Yuhiro
TOYA, Ryohei
SUEYOSHI, Masuo
Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title_full Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title_fullStr Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title_full_unstemmed Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title_short Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan
title_sort antimicrobial resistance profiles and phylogenetic groups of escherichia coli isolated from healthy thoroughbred racehorses in japan
topic Full Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750643/
https://www.ncbi.nlm.nih.gov/pubmed/33376444
http://dx.doi.org/10.1294/jes.31.85
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