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Positive/negative ion-switching-based LC–MS/MS method for quantification of cytosine derivatives produced by the TET-family 5-methylcytosine dioxygenases

Cytosine methylation at carbon-5 (5mC) in DNA plays crucial roles in epigenetic transcriptional regulation during metazoan development. The iron (II), 2-oxoglutarate-dependent Ten-Eleven Translocation (TET)-family dioxygenases initiate active demethylation of 5mC. TET2 oxidizes 5mC in nucleic acids...

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Detalles Bibliográficos
Autores principales:	Dey, Aninda Sundar, Ayon, Navid J, Bhattacharya, Chayan, Gutheil, William G, Mukherji, Mridul
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751006/ https://www.ncbi.nlm.nih.gov/pubmed/33376805 http://dx.doi.org/10.1093/biomethods/bpaa019

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