Cargando…
Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension
Pulmonary hypertension (PH) is a life-threatening disease that often involves vascular remodeling. Although pulmonary arterial smooth muscle cells (PASMCs) are the primary participants in vascular remodeling, their biological role is not entirely clear. The present study analyzed the role of enhance...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751464/ https://www.ncbi.nlm.nih.gov/pubmed/33313943 http://dx.doi.org/10.3892/mmr.2020.11768 |
_version_ | 1783625672554446848 |
---|---|
author | Wang, Ying Huang, Xiao-Xi Leng, Dong Li, Ji-Feng Liang, Yan Jiang, Tao |
author_facet | Wang, Ying Huang, Xiao-Xi Leng, Dong Li, Ji-Feng Liang, Yan Jiang, Tao |
author_sort | Wang, Ying |
collection | PubMed |
description | Pulmonary hypertension (PH) is a life-threatening disease that often involves vascular remodeling. Although pulmonary arterial smooth muscle cells (PASMCs) are the primary participants in vascular remodeling, their biological role is not entirely clear. The present study analyzed the role of enhancer of zeste homolog 2 (EZH2) in vascular remodeling of PH by investigating the behavior of PASMCs. The expression levels of EZH2 in PASMCs in chronic thromboembolic pulmonary hypertension (CTEPH), a type of PH, were detected. The role of EZH2 in PASMC migration was investigated by wound-healing assay following overexpression and knockdown. Functional enrichment analysis of the whole-genome expression profiles of PASMCs with EZH2 overexpression was performed using an mRNA Human Gene Expression Microarray. Quantitative (q)PCR was performed to confirm the results of the microarray. EZH2 expression levels increased in CTEPH cell models. The overexpression of EZH2 enhanced PASMC migration compared with control conditions. Functional enrichment analysis of the differentially expressed genes following EZH2 overexpression indicated a strong link between EZH2 and the immune inflammatory response and oxidoreductase activity in PASMCs. mRNA expression levels of superoxide dismutase 3 were verified by qPCR. The results suggested that EZH2 was involved in the migration of PASMCs in PH, and may serve as a potential target for the treatment of PH. |
format | Online Article Text |
id | pubmed-7751464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-77514642020-12-28 Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension Wang, Ying Huang, Xiao-Xi Leng, Dong Li, Ji-Feng Liang, Yan Jiang, Tao Mol Med Rep Articles Pulmonary hypertension (PH) is a life-threatening disease that often involves vascular remodeling. Although pulmonary arterial smooth muscle cells (PASMCs) are the primary participants in vascular remodeling, their biological role is not entirely clear. The present study analyzed the role of enhancer of zeste homolog 2 (EZH2) in vascular remodeling of PH by investigating the behavior of PASMCs. The expression levels of EZH2 in PASMCs in chronic thromboembolic pulmonary hypertension (CTEPH), a type of PH, were detected. The role of EZH2 in PASMC migration was investigated by wound-healing assay following overexpression and knockdown. Functional enrichment analysis of the whole-genome expression profiles of PASMCs with EZH2 overexpression was performed using an mRNA Human Gene Expression Microarray. Quantitative (q)PCR was performed to confirm the results of the microarray. EZH2 expression levels increased in CTEPH cell models. The overexpression of EZH2 enhanced PASMC migration compared with control conditions. Functional enrichment analysis of the differentially expressed genes following EZH2 overexpression indicated a strong link between EZH2 and the immune inflammatory response and oxidoreductase activity in PASMCs. mRNA expression levels of superoxide dismutase 3 were verified by qPCR. The results suggested that EZH2 was involved in the migration of PASMCs in PH, and may serve as a potential target for the treatment of PH. D.A. Spandidos 2021-02 2020-12-10 /pmc/articles/PMC7751464/ /pubmed/33313943 http://dx.doi.org/10.3892/mmr.2020.11768 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Ying Huang, Xiao-Xi Leng, Dong Li, Ji-Feng Liang, Yan Jiang, Tao Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title | Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title_full | Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title_fullStr | Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title_full_unstemmed | Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title_short | Effect of EZH2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
title_sort | effect of ezh2 on pulmonary artery smooth muscle cell migration in pulmonary hypertension |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751464/ https://www.ncbi.nlm.nih.gov/pubmed/33313943 http://dx.doi.org/10.3892/mmr.2020.11768 |
work_keys_str_mv | AT wangying effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension AT huangxiaoxi effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension AT lengdong effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension AT lijifeng effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension AT liangyan effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension AT jiangtao effectofezh2onpulmonaryarterysmoothmusclecellmigrationinpulmonaryhypertension |