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Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse
PURPOSE: The present study investigates the effects of myricitrin and solid lipid nanoparticle (SLN) containing myricitrin on the reproductive system of type 2 diabetic male mice. MATERIALS AND METHODS: In this experimental study, SLN containing myricitrin was prepared by the cold homogenization met...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Sexual Medicine and Andrology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752505/ https://www.ncbi.nlm.nih.gov/pubmed/32009314 http://dx.doi.org/10.5534/wjmh.190010 |
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author | Oroojan, Ali Akbar Ahangarpour, Akram Paknejad, Babak Zareian, Parvin Hami, Zahra Abtahi, Seyed Reza |
author_facet | Oroojan, Ali Akbar Ahangarpour, Akram Paknejad, Babak Zareian, Parvin Hami, Zahra Abtahi, Seyed Reza |
author_sort | Oroojan, Ali Akbar |
collection | PubMed |
description | PURPOSE: The present study investigates the effects of myricitrin and solid lipid nanoparticle (SLN) containing myricitrin on the reproductive system of type 2 diabetic male mice. MATERIALS AND METHODS: In this experimental study, SLN containing myricitrin was prepared by the cold homogenization method. Then, 90 adult male Naval Medical Research Institute mice were divided into 9 groups (n=10): control, vehicle, diabetic, diabetic+myricitrin or SLN containing myricitrin 1, 3, and 10 mg/kg. Diabetes was induced by streptozotocin (65 mg/kg) 15 minutes after nicotinamide (120 mg/kg) injection. Myricitrin and SLN containing myricitrin administered during 1 month. At the 34th days of the experiment, plasma and tissue samples were taken for experimental assessments. RESULTS: Testis weight and volume decreased in the diabetic group. These variables increased in diabetic treated mice by a high dose of myricitrin or all doses of SLN containing myricitrin (p<0.05). Total antioxidant capacity and superoxide dismutase levels decreased in diabetic mice, and administration of myricitrin 10 mg/kg or all doses of SLN containing myricitrin increased them (p<0.05). Luteinizing hormone, Follicle-stimulating hormone, testosterone, and sperm count decreased in the diabetic group, treatment with a high dose of myricitrin or all doses of SLN containing myricitrin recovered them (p<0.05). Diabetes induced vacuoles and apoptosis in testicular cells, meanwhile myricitrin and SLN containing myricitrin improved them (p<0.05). CONCLUSIONS: Diabetes induced reproductive problem via increased oxidative stress and decrease antioxidant capacity, administration of myricitrin or SLN containing myricitrin improved them. Further, SLN containing myricitrin was more potent than myricitrin. |
format | Online Article Text |
id | pubmed-7752505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Korean Society for Sexual Medicine and Andrology |
record_format | MEDLINE/PubMed |
spelling | pubmed-77525052021-01-05 Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse Oroojan, Ali Akbar Ahangarpour, Akram Paknejad, Babak Zareian, Parvin Hami, Zahra Abtahi, Seyed Reza World J Mens Health Original Article PURPOSE: The present study investigates the effects of myricitrin and solid lipid nanoparticle (SLN) containing myricitrin on the reproductive system of type 2 diabetic male mice. MATERIALS AND METHODS: In this experimental study, SLN containing myricitrin was prepared by the cold homogenization method. Then, 90 adult male Naval Medical Research Institute mice were divided into 9 groups (n=10): control, vehicle, diabetic, diabetic+myricitrin or SLN containing myricitrin 1, 3, and 10 mg/kg. Diabetes was induced by streptozotocin (65 mg/kg) 15 minutes after nicotinamide (120 mg/kg) injection. Myricitrin and SLN containing myricitrin administered during 1 month. At the 34th days of the experiment, plasma and tissue samples were taken for experimental assessments. RESULTS: Testis weight and volume decreased in the diabetic group. These variables increased in diabetic treated mice by a high dose of myricitrin or all doses of SLN containing myricitrin (p<0.05). Total antioxidant capacity and superoxide dismutase levels decreased in diabetic mice, and administration of myricitrin 10 mg/kg or all doses of SLN containing myricitrin increased them (p<0.05). Luteinizing hormone, Follicle-stimulating hormone, testosterone, and sperm count decreased in the diabetic group, treatment with a high dose of myricitrin or all doses of SLN containing myricitrin recovered them (p<0.05). Diabetes induced vacuoles and apoptosis in testicular cells, meanwhile myricitrin and SLN containing myricitrin improved them (p<0.05). CONCLUSIONS: Diabetes induced reproductive problem via increased oxidative stress and decrease antioxidant capacity, administration of myricitrin or SLN containing myricitrin improved them. Further, SLN containing myricitrin was more potent than myricitrin. Korean Society for Sexual Medicine and Andrology 2021-01 2019-12-06 /pmc/articles/PMC7752505/ /pubmed/32009314 http://dx.doi.org/10.5534/wjmh.190010 Text en Copyright © 2021 Korean Society for Sexual Medicine and Andrology http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Oroojan, Ali Akbar Ahangarpour, Akram Paknejad, Babak Zareian, Parvin Hami, Zahra Abtahi, Seyed Reza Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title | Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title_full | Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title_fullStr | Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title_full_unstemmed | Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title_short | Effects of Myricitrin and Solid Lipid Nanoparticle-Containing Myricitrin on Reproductive System Disorders Induced by Diabetes in Male Mouse |
title_sort | effects of myricitrin and solid lipid nanoparticle-containing myricitrin on reproductive system disorders induced by diabetes in male mouse |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752505/ https://www.ncbi.nlm.nih.gov/pubmed/32009314 http://dx.doi.org/10.5534/wjmh.190010 |
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