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Impact of contact lens wear on epithelial alterations in keratoconus

PURPOSE: The purpose of this study was to characterize the central epithelial thickness (CET) of penetrating keratoplasty corneal specimens obtained from patients with keratoconus (KC) and correlate the histological patterns with their clinical history. METHODS: Ex vivo histological imaging was perf...

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Autores principales: Sorbara, Luigina, Lopez, Jose Carlos Lopez, Gorbet, Maud, Bizheva, Kostadinka, Lamarca, Jose Mateu, Pastor, José-Carlos, Maldonado López, Miguel José, Hileeto, Denise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752984/
https://www.ncbi.nlm.nih.gov/pubmed/32376120
http://dx.doi.org/10.1016/j.optom.2020.02.005
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author Sorbara, Luigina
Lopez, Jose Carlos Lopez
Gorbet, Maud
Bizheva, Kostadinka
Lamarca, Jose Mateu
Pastor, José-Carlos
Maldonado López, Miguel José
Hileeto, Denise
author_facet Sorbara, Luigina
Lopez, Jose Carlos Lopez
Gorbet, Maud
Bizheva, Kostadinka
Lamarca, Jose Mateu
Pastor, José-Carlos
Maldonado López, Miguel José
Hileeto, Denise
author_sort Sorbara, Luigina
collection PubMed
description PURPOSE: The purpose of this study was to characterize the central epithelial thickness (CET) of penetrating keratoplasty corneal specimens obtained from patients with keratoconus (KC) and correlate the histological patterns with their clinical history. METHODS: Ex vivo histological imaging was performed to measure CET and total corneal thickness (TCT) in 56 patients with KC. Microscopic slides from penetrating keratoplasty corneal specimens, stained with hematoxylin and eosin were evaluated using bright field microscopy. CET and TCT were measured, and morphological features were studied. Clinical history regarding duration of KC prior to surgery and length of and tolerance to contact lens wear were compared and analyzed. RESULTS: The microscopic slides of all patients available for follow up (n = 48) were analyzed and CET and TCT were measured. The histological evaluation revealed 3 distinctive epithelial patterns. Pattern 1 with central hypertrophic and hydropic changes (n = 19) measured 70.89 ± 25.88 μm in CET and 308.63 ± 100.74 μm in TCT; Pattern 2 (n = 14) had not changed, similar to normal epithelium CET and TCT measuring 36.5 ± 7.02 μm and 260.14 ± 87.93 μm respectively. Pattern 3 (n = 15) demonstrated thinner central epithelium characterized by atrophy and focal hydropic changes measuring 19.93 ± 4.60 μm and 268.00 ± 79.39 μm in CET and TCT respectively (all p < 0.0001). The presence of Pattern 2 characterized by similar to normal CET was correlated with the duration of the condition (R = 0.600, p = 0.030). There was a significant difference in the length of CL wear comparing those with patterns 1 and 2 versus 3 (least no. of CL years) (p = 0.05 and p = 0.33 respectivelly). CONCLUSIONS: Patients with advanced disease have various central corneal epithelial changes detected with histology. Although each central epithelial pattern type was distinctive comparing the 3 patterns, there was no correlation with years of CL wear but only with the duration of the condition.
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spelling pubmed-77529842020-12-23 Impact of contact lens wear on epithelial alterations in keratoconus Sorbara, Luigina Lopez, Jose Carlos Lopez Gorbet, Maud Bizheva, Kostadinka Lamarca, Jose Mateu Pastor, José-Carlos Maldonado López, Miguel José Hileeto, Denise J Optom Original Article PURPOSE: The purpose of this study was to characterize the central epithelial thickness (CET) of penetrating keratoplasty corneal specimens obtained from patients with keratoconus (KC) and correlate the histological patterns with their clinical history. METHODS: Ex vivo histological imaging was performed to measure CET and total corneal thickness (TCT) in 56 patients with KC. Microscopic slides from penetrating keratoplasty corneal specimens, stained with hematoxylin and eosin were evaluated using bright field microscopy. CET and TCT were measured, and morphological features were studied. Clinical history regarding duration of KC prior to surgery and length of and tolerance to contact lens wear were compared and analyzed. RESULTS: The microscopic slides of all patients available for follow up (n = 48) were analyzed and CET and TCT were measured. The histological evaluation revealed 3 distinctive epithelial patterns. Pattern 1 with central hypertrophic and hydropic changes (n = 19) measured 70.89 ± 25.88 μm in CET and 308.63 ± 100.74 μm in TCT; Pattern 2 (n = 14) had not changed, similar to normal epithelium CET and TCT measuring 36.5 ± 7.02 μm and 260.14 ± 87.93 μm respectively. Pattern 3 (n = 15) demonstrated thinner central epithelium characterized by atrophy and focal hydropic changes measuring 19.93 ± 4.60 μm and 268.00 ± 79.39 μm in CET and TCT respectively (all p < 0.0001). The presence of Pattern 2 characterized by similar to normal CET was correlated with the duration of the condition (R = 0.600, p = 0.030). There was a significant difference in the length of CL wear comparing those with patterns 1 and 2 versus 3 (least no. of CL years) (p = 0.05 and p = 0.33 respectivelly). CONCLUSIONS: Patients with advanced disease have various central corneal epithelial changes detected with histology. Although each central epithelial pattern type was distinctive comparing the 3 patterns, there was no correlation with years of CL wear but only with the duration of the condition. Elsevier 2021 2020-05-04 /pmc/articles/PMC7752984/ /pubmed/32376120 http://dx.doi.org/10.1016/j.optom.2020.02.005 Text en © 2020 Spanish General Council of Optometry. Published by Elsevier España, S.L.U. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Sorbara, Luigina
Lopez, Jose Carlos Lopez
Gorbet, Maud
Bizheva, Kostadinka
Lamarca, Jose Mateu
Pastor, José-Carlos
Maldonado López, Miguel José
Hileeto, Denise
Impact of contact lens wear on epithelial alterations in keratoconus
title Impact of contact lens wear on epithelial alterations in keratoconus
title_full Impact of contact lens wear on epithelial alterations in keratoconus
title_fullStr Impact of contact lens wear on epithelial alterations in keratoconus
title_full_unstemmed Impact of contact lens wear on epithelial alterations in keratoconus
title_short Impact of contact lens wear on epithelial alterations in keratoconus
title_sort impact of contact lens wear on epithelial alterations in keratoconus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7752984/
https://www.ncbi.nlm.nih.gov/pubmed/32376120
http://dx.doi.org/10.1016/j.optom.2020.02.005
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