Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing

Translation regulation is a fundamental step in gene regulation with critical roles in neurodevelopment. Here, we describe three protocols to calculate the ribosomal-engagement levels of the transcriptome from in vitro-derived neuronal cells. The protocols described here include enrichment of in vit...

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Detalles Bibliográficos
Autores principales: Rodrigues, Deivid Carvalho, Mufteev, Marat, Ellis, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753199/
https://www.ncbi.nlm.nih.gov/pubmed/33364619
http://dx.doi.org/10.1016/j.xpro.2020.100229

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