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Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network

The role of angiogenesis in tumor progression has been recognized as one of the hallmarks of cancer, but the mechanism of its action remains unclear. Inflammatory markers serum amyloid A (SAA) and C-reactive protein (CRP) are proposed to play causal roles in the development of various disorders, inc...

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Autores principales: Liu, Dan, Chen, Yonghe, Wang, Yunxiu, Lei, Mangjuan, Chen, Lin, Liang, Rongliang, Cheng, Zhaomin, Shi, Wen, Wang, Huimin, Lin, Li, Wang, Lina, Lin, Fujia, Lin, Haibiao, Liu, Wanli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753214/
https://www.ncbi.nlm.nih.gov/pubmed/33364190
http://dx.doi.org/10.3389/fonc.2020.576207
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author Liu, Dan
Chen, Yonghe
Wang, Yunxiu
Lei, Mangjuan
Chen, Lin
Liang, Rongliang
Cheng, Zhaomin
Shi, Wen
Wang, Huimin
Lin, Li
Wang, Lina
Lin, Fujia
Lin, Haibiao
Liu, Wanli
author_facet Liu, Dan
Chen, Yonghe
Wang, Yunxiu
Lei, Mangjuan
Chen, Lin
Liang, Rongliang
Cheng, Zhaomin
Shi, Wen
Wang, Huimin
Lin, Li
Wang, Lina
Lin, Fujia
Lin, Haibiao
Liu, Wanli
author_sort Liu, Dan
collection PubMed
description The role of angiogenesis in tumor progression has been recognized as one of the hallmarks of cancer, but the mechanism of its action remains unclear. Inflammatory markers serum amyloid A (SAA) and C-reactive protein (CRP) are proposed to play causal roles in the development of various disorders, including malignancies. Previously, we identified the complex of CRP and SAA (CRP-SAA) with diagnostic and prognostic value better than either one of them in the serum of lung cancer patients. In this study, we further explored the stimulation function of CRP-SAA on angiogenesis and inflammation. To explore possible mechanisms, microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database and multi-bioinformatics analysis revealed that THP-1 and human umbilical vein endothelial cells (HUVECs) responded to SAA stimulation with upregulation of two pro-angiogenic cytokines in common, i.e., C-X-C motif ligand 6 (CXCL6) and CXCL8, which were validated by subsequent experiments in vitro. CRP had weak effects as a single stimulus, but it can efficiently potentiate the SAA induction of cytokines, which was stronger than the sum of the both (P < 0.001). The synergistical effect of the combination of CRP and SAA enhanced HUVECs transwell and constricted morphology by upregulating the pro-angiogenic genes. These results indicated that the binding of CRP and SAA acted synergistically in pro-angiogenesis by increasing inflammation and inducing vascular network.
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spelling pubmed-77532142020-12-23 Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network Liu, Dan Chen, Yonghe Wang, Yunxiu Lei, Mangjuan Chen, Lin Liang, Rongliang Cheng, Zhaomin Shi, Wen Wang, Huimin Lin, Li Wang, Lina Lin, Fujia Lin, Haibiao Liu, Wanli Front Oncol Oncology The role of angiogenesis in tumor progression has been recognized as one of the hallmarks of cancer, but the mechanism of its action remains unclear. Inflammatory markers serum amyloid A (SAA) and C-reactive protein (CRP) are proposed to play causal roles in the development of various disorders, including malignancies. Previously, we identified the complex of CRP and SAA (CRP-SAA) with diagnostic and prognostic value better than either one of them in the serum of lung cancer patients. In this study, we further explored the stimulation function of CRP-SAA on angiogenesis and inflammation. To explore possible mechanisms, microarray datasets were downloaded from the Gene Expression Omnibus (GEO) database and multi-bioinformatics analysis revealed that THP-1 and human umbilical vein endothelial cells (HUVECs) responded to SAA stimulation with upregulation of two pro-angiogenic cytokines in common, i.e., C-X-C motif ligand 6 (CXCL6) and CXCL8, which were validated by subsequent experiments in vitro. CRP had weak effects as a single stimulus, but it can efficiently potentiate the SAA induction of cytokines, which was stronger than the sum of the both (P < 0.001). The synergistical effect of the combination of CRP and SAA enhanced HUVECs transwell and constricted morphology by upregulating the pro-angiogenic genes. These results indicated that the binding of CRP and SAA acted synergistically in pro-angiogenesis by increasing inflammation and inducing vascular network. Frontiers Media S.A. 2020-12-08 /pmc/articles/PMC7753214/ /pubmed/33364190 http://dx.doi.org/10.3389/fonc.2020.576207 Text en Copyright © 2020 Liu, Chen, Wang, Lei, Chen, Liang, Cheng, Shi, Wang, Lin, Wang, Lin, Lin and Liu http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Liu, Dan
Chen, Yonghe
Wang, Yunxiu
Lei, Mangjuan
Chen, Lin
Liang, Rongliang
Cheng, Zhaomin
Shi, Wen
Wang, Huimin
Lin, Li
Wang, Lina
Lin, Fujia
Lin, Haibiao
Liu, Wanli
Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title_full Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title_fullStr Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title_full_unstemmed Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title_short Combination of Serum Amyloid A and C-Reactive Protein Exhibit Synergistic Effect in Angiogenesis by Inducing Inflammation and Vascular Network
title_sort combination of serum amyloid a and c-reactive protein exhibit synergistic effect in angiogenesis by inducing inflammation and vascular network
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753214/
https://www.ncbi.nlm.nih.gov/pubmed/33364190
http://dx.doi.org/10.3389/fonc.2020.576207
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