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Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models

Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial mode...

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Autores principales: Hedegaard, Anne, Stodolak, Szymon, James, William S., Cowley, Sally A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753623/
https://www.ncbi.nlm.nih.gov/pubmed/33363548
http://dx.doi.org/10.3389/fimmu.2020.614972
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author Hedegaard, Anne
Stodolak, Szymon
James, William S.
Cowley, Sally A.
author_facet Hedegaard, Anne
Stodolak, Szymon
James, William S.
Cowley, Sally A.
author_sort Hedegaard, Anne
collection PubMed
description Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models.
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spelling pubmed-77536232020-12-23 Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models Hedegaard, Anne Stodolak, Szymon James, William S. Cowley, Sally A. Front Immunol Immunology Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models. Frontiers Media S.A. 2020-12-08 /pmc/articles/PMC7753623/ /pubmed/33363548 http://dx.doi.org/10.3389/fimmu.2020.614972 Text en Copyright © 2020 Hedegaard, Stodolak, James and Cowley http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hedegaard, Anne
Stodolak, Szymon
James, William S.
Cowley, Sally A.
Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title_full Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title_fullStr Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title_full_unstemmed Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title_short Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models
title_sort honing the double-edged sword: improving human ipsc-microglia models
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753623/
https://www.ncbi.nlm.nih.gov/pubmed/33363548
http://dx.doi.org/10.3389/fimmu.2020.614972
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