Cargando…
Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection
Anti‐inflammatory and immune‐modulatory therapies have been proposed for the treatment of COVID‐19 and its most serious complications. Among others, the use of mesenchymal stromal cells (MSCs) is under investigation given their well‐documented anti‐inflammatory and immunomodulatory properties. Howev...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753681/ https://www.ncbi.nlm.nih.gov/pubmed/33188579 http://dx.doi.org/10.1002/sctm.20-0385 |
_version_ | 1783626062053244928 |
---|---|
author | Avanzini, Maria A. Mura, Manuela Percivalle, Elena Bastaroli, Francesca Croce, Stefania Valsecchi, Chiara Lenta, Elisa Nykjaer, Giulia Cassaniti, Irene Bagnarino, Jessica Baldanti, Fausto Zecca, Marco Comoli, Patrizia Gnecchi, Massimiliano |
author_facet | Avanzini, Maria A. Mura, Manuela Percivalle, Elena Bastaroli, Francesca Croce, Stefania Valsecchi, Chiara Lenta, Elisa Nykjaer, Giulia Cassaniti, Irene Bagnarino, Jessica Baldanti, Fausto Zecca, Marco Comoli, Patrizia Gnecchi, Massimiliano |
author_sort | Avanzini, Maria A. |
collection | PubMed |
description | Anti‐inflammatory and immune‐modulatory therapies have been proposed for the treatment of COVID‐19 and its most serious complications. Among others, the use of mesenchymal stromal cells (MSCs) is under investigation given their well‐documented anti‐inflammatory and immunomodulatory properties. However, some critical issues regarding the possibility that MSCs could be infected by the virus have been raised. Angiotensin‐converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) are the main host cell factors for the severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2), entry, but so far it is unclear if human MSCs do or do not express these two proteins. To elucidate these important aspects, we evaluated if human MSCs from both fetal and adult tissues constitutively express ACE2 and TMPRSS2 and, most importantly, if they can be infected by SARS‐CoV‐2. We evaluated human MSCs derived from amnios, cord blood, cord tissue, adipose tissue, and bone marrow. ACE2 and TMPRSS2 were expressed by the SARS‐CoV‐2‐permissive human pulmonary Calu‐3 cell line but not by all the MSCs tested. MSCs were then exposed to SARS‐CoV‐2 wild strain without evidence of cytopathic effect. Moreover, we also excluded that the MSCs could be infected without showing lytic effects since their conditioned medium after SARS‐CoV‐2 exposure did not contain viral particles. Our data, demonstrating that MSCs derived from different human tissues are not permissive to SARS‐CoV‐2 infection, support the safety of MSCs as potential therapy for COVID‐19. |
format | Online Article Text |
id | pubmed-7753681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-77536812020-12-22 Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection Avanzini, Maria A. Mura, Manuela Percivalle, Elena Bastaroli, Francesca Croce, Stefania Valsecchi, Chiara Lenta, Elisa Nykjaer, Giulia Cassaniti, Irene Bagnarino, Jessica Baldanti, Fausto Zecca, Marco Comoli, Patrizia Gnecchi, Massimiliano Stem Cells Transl Med Tissue‐specific Progenitor and Stem Cells Anti‐inflammatory and immune‐modulatory therapies have been proposed for the treatment of COVID‐19 and its most serious complications. Among others, the use of mesenchymal stromal cells (MSCs) is under investigation given their well‐documented anti‐inflammatory and immunomodulatory properties. However, some critical issues regarding the possibility that MSCs could be infected by the virus have been raised. Angiotensin‐converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) are the main host cell factors for the severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2), entry, but so far it is unclear if human MSCs do or do not express these two proteins. To elucidate these important aspects, we evaluated if human MSCs from both fetal and adult tissues constitutively express ACE2 and TMPRSS2 and, most importantly, if they can be infected by SARS‐CoV‐2. We evaluated human MSCs derived from amnios, cord blood, cord tissue, adipose tissue, and bone marrow. ACE2 and TMPRSS2 were expressed by the SARS‐CoV‐2‐permissive human pulmonary Calu‐3 cell line but not by all the MSCs tested. MSCs were then exposed to SARS‐CoV‐2 wild strain without evidence of cytopathic effect. Moreover, we also excluded that the MSCs could be infected without showing lytic effects since their conditioned medium after SARS‐CoV‐2 exposure did not contain viral particles. Our data, demonstrating that MSCs derived from different human tissues are not permissive to SARS‐CoV‐2 infection, support the safety of MSCs as potential therapy for COVID‐19. John Wiley & Sons, Inc. 2021-01-26 /pmc/articles/PMC7753681/ /pubmed/33188579 http://dx.doi.org/10.1002/sctm.20-0385 Text en © 2020 The Authors. stem cells translational medicine published by Wiley Periodicals LLC on behalf of AlphaMed Press. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Tissue‐specific Progenitor and Stem Cells Avanzini, Maria A. Mura, Manuela Percivalle, Elena Bastaroli, Francesca Croce, Stefania Valsecchi, Chiara Lenta, Elisa Nykjaer, Giulia Cassaniti, Irene Bagnarino, Jessica Baldanti, Fausto Zecca, Marco Comoli, Patrizia Gnecchi, Massimiliano Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title | Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title_full | Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title_fullStr | Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title_full_unstemmed | Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title_short | Human mesenchymal stromal cells do not express ACE2 and TMPRSS2 and are not permissive to SARS‐CoV‐2 infection |
title_sort | human mesenchymal stromal cells do not express ace2 and tmprss2 and are not permissive to sars‐cov‐2 infection |
topic | Tissue‐specific Progenitor and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753681/ https://www.ncbi.nlm.nih.gov/pubmed/33188579 http://dx.doi.org/10.1002/sctm.20-0385 |
work_keys_str_mv | AT avanzinimariaa humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT muramanuela humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT percivalleelena humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT bastarolifrancesca humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT crocestefania humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT valsecchichiara humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT lentaelisa humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT nykjaergiulia humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT cassanitiirene humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT bagnarinojessica humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT baldantifausto humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT zeccamarco humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT comolipatrizia humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection AT gnecchimassimiliano humanmesenchymalstromalcellsdonotexpressace2andtmprss2andarenotpermissivetosarscov2infection |