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Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay

BACKGROUND: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and...

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Autores principales: Man, Jin, Dong, Jiajia, Wang, Yilin, He, Leiliang, Yu, Songcheng, Yu, Fei, Wang, Jia, Tian, Yongmei, Liu, Lie, Han, Runping, Guo, Hongchao, Wu, Yongjun, Qu, Lingbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754089/
https://www.ncbi.nlm.nih.gov/pubmed/33363367
http://dx.doi.org/10.2147/IJN.S286317
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author Man, Jin
Dong, Jiajia
Wang, Yilin
He, Leiliang
Yu, Songcheng
Yu, Fei
Wang, Jia
Tian, Yongmei
Liu, Lie
Han, Runping
Guo, Hongchao
Wu, Yongjun
Qu, Lingbo
author_facet Man, Jin
Dong, Jiajia
Wang, Yilin
He, Leiliang
Yu, Songcheng
Yu, Fei
Wang, Jia
Tian, Yongmei
Liu, Lie
Han, Runping
Guo, Hongchao
Wu, Yongjun
Qu, Lingbo
author_sort Man, Jin
collection PubMed
description BACKGROUND: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF. METHODS: Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples. RESULTS: Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL(−1), and the limit of detection was 0.1 ng mL(−1). The linear range of the calibration curve for CEA was 0.5 to 160 ng mL(−1), and the limit of detection was 0.1 ng mL(−1). The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits. CONCLUSION: The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.
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spelling pubmed-77540892020-12-23 Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay Man, Jin Dong, Jiajia Wang, Yilin He, Leiliang Yu, Songcheng Yu, Fei Wang, Jia Tian, Yongmei Liu, Lie Han, Runping Guo, Hongchao Wu, Yongjun Qu, Lingbo Int J Nanomedicine Original Research BACKGROUND: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF. METHODS: Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples. RESULTS: Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL(−1), and the limit of detection was 0.1 ng mL(−1). The linear range of the calibration curve for CEA was 0.5 to 160 ng mL(−1), and the limit of detection was 0.1 ng mL(−1). The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits. CONCLUSION: The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis. Dove 2020-12-14 /pmc/articles/PMC7754089/ /pubmed/33363367 http://dx.doi.org/10.2147/IJN.S286317 Text en © 2020 Man et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Man, Jin
Dong, Jiajia
Wang, Yilin
He, Leiliang
Yu, Songcheng
Yu, Fei
Wang, Jia
Tian, Yongmei
Liu, Lie
Han, Runping
Guo, Hongchao
Wu, Yongjun
Qu, Lingbo
Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title_full Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title_fullStr Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title_full_unstemmed Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title_short Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay
title_sort simultaneous detection of vegf and cea by time-resolved chemiluminescence enzyme-linked aptamer assay
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754089/
https://www.ncbi.nlm.nih.gov/pubmed/33363367
http://dx.doi.org/10.2147/IJN.S286317
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