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Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds
[Image: see text] For metabolic engineering approaches, fast and reliable tools are required to precisely manipulate the expression of target genes. dCas9 can be fused via RNA scaffolds to trans-activator domains and thus regulate the gene expression when targeted to the promoter region of a gene. I...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754189/ https://www.ncbi.nlm.nih.gov/pubmed/33180466 http://dx.doi.org/10.1021/acssynbio.0c00214 |